Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Seffernick, J.L.; McTavish, H.; Osborne, J.P.; de Souza, M.L.; Sadowsky, M.J.; Wackett, L.P.
    Atrazine chlorohydrolase from Pseudomonas sp. strain ADP is a metalloenzyme (2002), Biochemistry, 41, 14430-14437.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.8 gene atzA, overexpression in Escherichia coli DH5alpha Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.8 1,10-phenanthroline complete inhibition, reversible by addition of Fe2+, Mn2+, or Co2+ Pseudomonas sp.
3.8.1.8 Cu2+ complete inhibition, not due to displacement of the native active site metal ion Fe2+ Pseudomonas sp.
3.8.1.8 Fe3+ complete inhibition, not due to displacement of the native active site metal ion Fe2+ Pseudomonas sp.
3.8.1.8 Ni2+ complete inhibition, not due to displacement of the native active site metal ion Fe2+ Pseudomonas sp.
3.8.1.8 oxalic acid reversible by addition of Fe2+, Mn2+, or Co2+ Pseudomonas sp.
3.8.1.8 Zn2+ complete inhibition, not due to displacement of the native active site metal ion Fe2+ Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.8.1.8 Co2+ activates Pseudomonas sp.
3.8.1.8 Fe2+ iron containing metalloenzyme, 1:1 metal to subunit stoichiometry, bound at the active site, activates Pseudomonas sp.
3.8.1.8 Mn2+ slightly stimulating Pseudomonas sp.
3.8.1.8 additional information influence of and reconstitution with metals Mn2+, Fe2+, and Co2+, overview Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.8 atrazine + H2O Pseudomonas sp. catabolic atrazine degradation pathway, overview, dechlorination 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.8 Pseudomonas sp.
-
 gene atzA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.8 recombinant from Escherichia coli Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.8 3.7
-
 about, purified recombinant enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.8 atrazine + H2O
-
 Pseudomonas sp. 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
 ir
3.8.1.8 atrazine + H2O catabolic atrazine degradation pathway, overview, dechlorination Pseudomonas sp. 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.8.1.8 AtzA
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.8 23
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.8 6.9
-
 assay at Pseudomonas sp.