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Literature summary extracted from

  • Nguyen, T.V.; Riggs, C.; Babovic-Vuksanovic, D.; Kim, Y.S.; Carpenter, J.F.; Burghardt, T.P.; Gregersen, N.; Vockley, J.
    Purification and characterization of two polymorphic variants of short chain acyl-CoA dehydrogenase reveal reduction of catalytic activity and stability of the Gly185Ser enzyme (2002), Biochemistry, 41, 11126-11133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.1 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.1 G185S 80% of wild-type kcat with butanoyl-CoA Homo sapiens
1.3.8.1 R147W 135% of wild-type kcat with butanoyl-CoA Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.1 0.0003
-
 butanoyl-CoA 32°C, pH 7.0 Homo sapiens
1.3.8.1 0.00045
-
 butanoyl-CoA 32°C, pH 7.0, R147W mutant Homo sapiens
1.3.8.1 0.00075
-
 butanoyl-CoA 32°C, pH 7.0, G185S mutant Homo sapiens
1.3.8.1 0.004
-
 hexanoyl-CoA 32°C, pH 7.0 Homo sapiens
1.3.8.1 0.019
-
 2-methylbutanoyl-CoA 32°C, pH 7.0 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.1 recombinant SCAD Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.1 5.9
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.1 2-methylbutanoyl-CoA + acceptor
-
 Homo sapiens 2-methyl-2-butenoyl-CoA + reduced acceptor
-
 r
1.3.8.1 butanoyl-CoA + acceptor
-
 Homo sapiens 2-butenoyl-CoA + reduced acceptor
-
 r
1.3.8.1 hexanoyl-CoA + acceptor
-
 Homo sapiens 2-hexenoyl-CoA + reduced acceptor
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.3.8.1 SCAD
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.8.1 45
-
 15% loss of activity after 3 h, wild-type SCAD coexpressed with GroEL Homo sapiens
1.3.8.1 45
-
 approx. 50% loss of activity after 3 h, R147W mutant expressed without GroEL Homo sapiens
1.3.8.1 45
-
 approx. 50% loss of activity after 3 h, wild-type expressed without GroEL Homo sapiens
1.3.8.1 45
-
 approx. 85% loss of activity after 3 h, G185S mutant expressed without GroEL Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.1 6
-
 2-methylbutanoyl-CoA 32°C, pH 7.0 Homo sapiens
1.3.8.1 9.6
-
 butanoyl-CoA 32°C, pH 7.0 Homo sapiens
1.3.8.1 10
-
 hexanoyl-CoA 32°C, pH 7.0 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.8.1 6 8 no loss of activity after 5 h below 37°C in the presence of less than 3% glycerol Homo sapiens