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Literature summary extracted from

  • Hara-Yokoyama, M.; Nagatsuka, Y.; Katsumata, O.; Irie, F.; Kontani, K.; Hoshino, S.; Katada, T.; Ono, Y.; Fujita-Yoshigaki, J.; Sugiya, H.; Furuyama, S.; Hirabayashi, Y.
    Complex gangliosides as cell surface inhibitors for the ecto-NAD+ glycohydrolase of CD38 (2001), Biochemistry, 40, 888-895.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.5 expression in THP-1 cells, selection of transfected cells by G418 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.5 b-series gangliosides
-
 Homo sapiens
3.2.2.5 GD1a exogenous ganglioside, slight inhibition, inhibition in vivo Homo sapiens
3.2.2.5 GD1b exogenous ganglioside, inhibition in vivo Homo sapiens
3.2.2.5 GD3 exogenous ganglioside, inhibition in vivo Homo sapiens
3.2.2.5 GM1b exogenous ganglioside, slight inhibition, inhibition in vivo Homo sapiens
3.2.2.5 GT1b exogenous ganglioside, inhibition in vivo, needs to be incorporated into the cell to inhibit CD38, preferably cis interaction, i.e. CD38 and GT1b are located on the same cell, the tandem sialic acid residues linked to the internal galactose of the gangliotetraose core are crucial to the inhibition Homo sapiens
3.2.2.5 additional information structure of the inhibiting gangliosides, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.5 membrane transmembrane protein with a catalytically active extracellular domain Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.5 NAD+ + H2O Homo sapiens
-
 ADP-ribose + nicotinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.5 Homo sapiens
-
 the extracellular domain of the leukocyte single transmembrane cell surface antigen CD38 shows NADase activity
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.5 NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ the enzyme can also hydrolyse NADP+ to yield phospho-ADP-ribose and nicotinamide, but more slowly Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.5 HL-60 cell leukemic cells, retinoic acid-treated Homo sapiens
-
3.2.2.5 leukocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.5 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.5 NAD+ + H2O
-
 Homo sapiens ADP-ribose + nicotinamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.5 CD38
-
 Homo sapiens
3.2.2.5 NADase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.5 37
-
 assay at Homo sapiens