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Literature summary extracted from

  • Loescher, S.; Burgdorf, T.; Buhrke, T.; Friedrich, B.; Dau, H.; Haumann, M.
    Non-standard structures of the Ni-Fe cofactor in the regulatory and the NAD-reducing hydrogenases from Ralstonia eutropha (2005), Biochem. Soc. Trans., 33, 25-27.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.1.2 additional information no inhibition by O2 Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.2 soluble
-
 Cupriavidus necator
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.2 CN- enzyme contains four cyanides in its active site, the Ni2+ bound one is responsible for the insensitivity towards oxygen, the active site is a (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO) centre, the CN- bound to the nickel ion can be irreversibly removed inducing enzyme inhibition by oxygen Cupriavidus necator
1.12.1.2 CO bound to the active site, the active site is a (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO) centre Cupriavidus necator
1.12.1.2 Fe2+ the active site is a (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO) centre Cupriavidus necator
1.12.1.2 Ni2+ the active site is a (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO) centre, H2 activation solely takes place on Ni2+ Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.2 H2 + NAD+ Cupriavidus necator enzyme provides reducing equivalents for CO2 fixation H+ + NADH
-
 r
1.12.1.2 additional information Cupriavidus necator enzyme regulation involves a regulatory hydrogenase RH which acts as a sensor for H2 content, interaction via signal cascade ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Cupriavidus necator
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+
-
 Cupriavidus necator H+ + NADH
-
 r
1.12.1.2 H2 + NAD+ enzyme provides reducing equivalents for CO2 fixation Cupriavidus necator H+ + NADH
-
 r
1.12.1.2 additional information enzyme regulation involves a regulatory hydrogenase RH which acts as a sensor for H2 content, interaction via signal cascade Cupriavidus necator ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.1.2 NAD-reducing hydrogenase
-
 Cupriavidus necator
1.12.1.2 SH
-
 Cupriavidus necator
1.12.1.2 soluble hydrogenase
-
 Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.2 additional information Ni-Fe cofactor, analysis of the non-standard structure of the Ni-Fe cofactor bound at the active site, interaction mechanism with H2, structural changes during reaction, reduction, and inactivation, the cofactor structure is (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO), overview Cupriavidus necator
1.12.1.2 NAD+
-
 Cupriavidus necator
1.12.1.2 NADH
-
 Cupriavidus necator