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Literature summary extracted from

  • Bonner, C.A.; Jensen, R.A.; Gander, J.E.; Keyhani, N.O.
    A core catalytic domain of the TyrA protein family: arogenate dehydrogenase from Synechocystis (2004), Biochem. J., 382, 279-291.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.78 expression in Escherichia coli Synechocystis sp. PCC 6803

General Stability

EC Number General Stability Organism
1.3.1.78 full activity is retained in repeated freeze-thaw cycles, when concentrations of 0.01 mg of protein per ml or more are maintained Synechocystis sp. PCC 6803

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.78 2',5'-ADP
-
 Synechocystis sp. PCC 6803
1.3.1.78 L-Tyr
-
 Synechocystis sp. PCC 6803

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.78 0.038
-
 NADP+ pH 8.6, 25°C Synechocystis sp. PCC 6803
1.3.1.78 0.331
-
 L-arogenate pH 8.6, 25°C Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.78 L-arogenate + NADP+ Chlorella sorokiniana enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ Porphyridium purpureum enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ Anabaena sp. enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ Synechocystis sp. enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ Fischerella sp. enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ Prochlorothrix hollandica enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ Synechocystis sp. PCC 6803 enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ Synechococcus elongatus PCC 6301 enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ Synechocystis sp. PCC 6902 enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.78 Anabaena sp.
-
 ATCC 29151
-
1.3.1.78 Chlorella sorokiniana
-
-
-
1.3.1.78 Fischerella sp.
-
 ATCC 29539
-
1.3.1.78 Porphyridium purpureum
-
-
-
1.3.1.78 Prochlorothrix hollandica
-
-
-
1.3.1.78 Synechococcus elongatus PCC 6301
-
-
-
1.3.1.78 Synechocystis sp.
-
-
-
1.3.1.78 Synechocystis sp. PCC 6803 P73906
-
-
1.3.1.78 Synechocystis sp. PCC 6902
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.78
-
 Synechocystis sp. PCC 6803

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.78 L-arogenate + NADP+ = L-tyrosine + NADPH + CO2 + H+ mechanism is probably steady-state random order Synechocystis sp. PCC 6803
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.78 0.0024
-
-
 Chlorella sorokiniana
1.3.1.78 0.0024
-
-
 Prochlorothrix hollandica
1.3.1.78 0.0078
-
-
 Anabaena sp.
1.3.1.78 0.0097
-
-
 Porphyridium purpureum
1.3.1.78 0.0109
-
-
 Fischerella sp.
1.3.1.78 0.0115
-
-
 Synechococcus elongatus PCC 6301
1.3.1.78 0.0145
-
-
 Synechocystis sp.
1.3.1.78 220
-
-
 Synechocystis sp. PCC 6803

Storage Stability

EC Number Storage Stability Organism
1.3.1.78 purified enzyme is less stable during storage at 4°C than when maintained frozen at -20°C or at -70°C Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.78 L-arogenate + NADP+ enzyme of the arogenate pathway Chlorella sorokiniana L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ enzyme of the arogenate pathway Porphyridium purpureum L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ enzyme of the arogenate pathway Anabaena sp. L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ enzyme of the arogenate pathway Synechocystis sp. L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ enzyme of the arogenate pathway Fischerella sp. L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ enzyme of the arogenate pathway Prochlorothrix hollandica L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ enzyme of the arogenate pathway Synechocystis sp. PCC 6803 L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ enzyme of the arogenate pathway Synechococcus elongatus PCC 6301 L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Chlorella sorokiniana L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Porphyridium purpureum L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Anabaena sp. L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Synechocystis sp. L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Fischerella sp. L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Prochlorothrix hollandica L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Synechocystis sp. PCC 6803 L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Synechococcus elongatus PCC 6301 L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ enzyme of the arogenate pathway Synechocystis sp. PCC 6902 L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Synechocystis sp. PCC 6902 L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 additional information no activity with prephenate and NAD+ Chlorella sorokiniana ?
-
 ?
1.3.1.78 additional information no activity with prephenate and NAD+ Porphyridium purpureum ?
-
 ?
1.3.1.78 additional information no activity with prephenate and NAD+ Anabaena sp. ?
-
 ?
1.3.1.78 additional information no activity with prephenate and NAD+ Synechocystis sp. ?
-
 ?
1.3.1.78 additional information no activity with prephenate and NAD+ Fischerella sp. ?
-
 ?
1.3.1.78 additional information no activity with prephenate and NAD+ Prochlorothrix hollandica ?
-
 ?
1.3.1.78 additional information no activity with prephenate and NAD+ Synechocystis sp. PCC 6803 ?
-
 ?
1.3.1.78 additional information no activity with prephenate and NAD+ Synechococcus elongatus PCC 6301 ?
-
 ?
1.3.1.78 additional information no activity with prephenate and NAD+ Synechocystis sp. PCC 6902 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.78 dimer
-
 Synechocystis sp. PCC 6803

Synonyms

EC Number Synonyms Comment Organism
1.3.1.78 TyrAa
-
 Chlorella sorokiniana
1.3.1.78 TyrAa
-
 Porphyridium purpureum
1.3.1.78 TyrAa
-
 Anabaena sp.
1.3.1.78 TyrAa
-
 Synechocystis sp.
1.3.1.78 TyrAa
-
 Fischerella sp.
1.3.1.78 TyrAa
-
 Prochlorothrix hollandica
1.3.1.78 TyrAa
-
 Synechocystis sp. PCC 6803
1.3.1.78 TyrAa
-
 Synechococcus elongatus PCC 6301

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.78 28 30
-
 Synechocystis sp. PCC 6803

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.78 28 42 28-30°C: optimum, 42°C: 50% of maximal activity Synechocystis sp. PCC 6803

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.78 8.3 8.8
-
 Synechocystis sp. PCC 6803

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.78 NADP+ no activity with NAD+ Chlorella sorokiniana
1.3.1.78 NADP+ no activity with NAD+ Porphyridium purpureum
1.3.1.78 NADP+ no activity with NAD+ Anabaena sp.
1.3.1.78 NADP+ no activity with NAD+ Synechocystis sp.
1.3.1.78 NADP+ no activity with NAD+ Fischerella sp.
1.3.1.78 NADP+ no activity with NAD+ Prochlorothrix hollandica
1.3.1.78 NADP+ no activity with NAD+ Synechocystis sp. PCC 6803
1.3.1.78 NADP+ no activity with NAD+ Synechococcus elongatus PCC 6301

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.78 0.078
-
 L-Tyr pH 8.6, 25°C, versus NADP+ Synechocystis sp. PCC 6803
1.3.1.78 0.089
-
 L-Tyr pH 8.6, 25°C, versus L-arogenate Synechocystis sp. PCC 6803