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Literature summary extracted from

  • Frebortova, J.; Fraaije, M.W.; Galuszka, P.; Sebela, M.; Pec, P.; Hrbac, J.; Novak, O.; Bilyeu, K.D.; English, J.T.; Frebort, I.
    Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors (2004), Biochem. J., 380, 121-130.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.12 expression of ZmCKX1 in Pichia pastoris Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.12 additional information
-
 additional information kinetics, redox potential of electron acceptors Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.12 N6-(2-isopentenyl)adenine + FAD Zea mays the natural terminal electron acceptor probably is a p-quinone or a similar compound, high substrate specificity for N6-(2-isopentenyl)adenine adenine + 3-methylbut-2-enal + FADH2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.12 Zea mays Q9T0N8 enzyme and gene ZmCKX1
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.99.12 N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor reaction scheme and mechanism, reduction of N6-(2-isopentenyl)adenine is rapidly performed, the reaction with O2 is slowly Zea mays
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.12 additional information substrate specificity with cytokinins, overiew Zea mays ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + 1,4-naphthoquinone
-
 Zea mays adenine + 3-methylbut-2-enal + reduced 1,4-naphthoquinone
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone i.e. coenzyme Q0 Zea mays adenine + 3-methylbut-2-enal + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol
-
 Zea mays adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + caffeic acid
-
 Zea mays adenine + 3-methylbut-2-enal + ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + coenzyme Q1
-
 Zea mays adenine + 3-methylbut-2-enal + reduced coenzyme Q1
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + cytochrome c
-
 Zea mays adenine + 3-methylbut-2-enal + reduced cytochrome c
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + daphnoretin
-
 Zea mays adenine + 3-methylbut-2-enal + ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + FAD
-
 Zea mays adenine + 3-methylbut-2-enal + FADH2
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + FAD the natural terminal electron acceptor probably is a p-quinone or a similar compound, high substrate specificity for N6-(2-isopentenyl)adenine Zea mays adenine + 3-methylbut-2-enal + FADH2
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + o-coumaric acid
-
 Zea mays adenine + 3-methylbut-2-enal + ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + O2 oxidative degradation of cytokinins, slow reaction, low substrate specificity Zea mays adenine + 3-methylbut-2-enal + H2O2
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + plantacyanine electron acceptor from spinach Zea mays adenine + 3-methylbut-2-enal + reduced plantacyanine
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + rosmarinic acid
-
 Zea mays adenine + 3-methylbut-2-enal + ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + scopoletin
-
 Zea mays adenine + 3-methylbut-2-enal + ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + spinach plantacyanine
-
 Zea mays adenine + 3-methylbut-2-enal + reduced spinach plantacyanine
-
 r
1.5.99.12 N6-(2-isopentenyl)adenine + vitamin K1
-
 Zea mays adenine + 3-methylbut-2-enal + reduced vitamin K1
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + vitamin K3
-
 Zea mays adenine + 3-methylbut-2-enal + reduced vitamin K3
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenosine + FAD
-
 Zea mays adenosine + 3-methylbut-2-enal + FADH2
-
 ?
1.5.99.12 zeatin + FAD
-
 Zea mays ? + FADH2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.12 CKX
-
 Zea mays
1.5.99.12 cytokinin oxidase/dehydrogenase
-
 Zea mays
1.5.99.12 More enzyme belongs to the plant CKX family Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.99.12 additional information
-
 additional information at pH above pH 6.5 polymerization of the quinone acceptors occur Zea mays
1.5.99.12 0.1
-
 O2 below, pH 8.0, recombinant enzyme Zea mays
1.5.99.12 0.3
-
 caffeic acid pH 6.5, recombinant enzyme Zea mays
1.5.99.12 0.3
-
 o-coumaric acid pH 6.5, recombinant enzyme Zea mays
1.5.99.12 0.4
-
 rosmarinic acid pH 6.5, recombinant enzyme Zea mays
1.5.99.12 0.6
-
 O2 pH 6.5, recombinant enzyme Zea mays
1.5.99.12 0.8
-
 cytochrome c pH 6.5, recombinant enzyme Zea mays
1.5.99.12 0.9
-
 spinach plantacyanine pH 6.5, recombinant enzyme Zea mays
1.5.99.12 1
-
 daphnoretin pH 6.5, recombinant enzyme Zea mays
1.5.99.12 1.4
-
 scopoletin pH 6.5, recombinant enzyme Zea mays
1.5.99.12 2.3
-
 vitamin K1 pH 6.5, recombinant enzyme Zea mays
1.5.99.12 85.4
-
 vitamin K3 pH 6.5, recombinant enzyme Zea mays
1.5.99.12 123.1
-
 coenzyme Q1 pH 6.5, recombinant enzyme Zea mays
1.5.99.12 129.8
-
 N6-(2-isopentenyl)adenosine pH 6.5, recombinant enzyme, with cofactor 2,3-dimethoxy-5-methyl-1,4-benzoquinone Zea mays
1.5.99.12 143.6
-
 2,3-dimethoxy-5-methyl-1,4-benzoquinone pH 6.5, recombinant enzyme Zea mays
1.5.99.12 143.6
-
 N6-(2-isopentenyl)adenine pH 6.5, recombinant enzyme, with cofactor 2,3-dimethoxy-5-methyl-1,4-benzoquinone Zea mays
1.5.99.12 169.6
-
 1,4-Naphthoquinone pH 6.5, recombinant enzyme Zea mays
1.5.99.12 178.4
-
 2,6-dichlorophenolindophenol pH 6.5, recombinant enzyme Zea mays
1.5.99.12 278
-
 2,6-dichlorophenolindophenol pH 8.0, recombinant enzyme Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.12 additional information
-
 substrate-dependent pH-profile, overview Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.12 FAD covalently bound, redox potential analysis Zea mays
1.5.99.12 additional information enzyme shows a preference for quinones as electron acceptors Zea mays