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Literature summary extracted from

  • Watanabe, T.; Ariga, Y.; Sato, U.; Toratani, T.; Hashimoto, M.; Nikaidou, N.; Kezuka, Y.; Nonaka, T.; Sugiyama, J.
    Aromatic residues within the substrate-binding cleft of Bacillus circulans chitinase A1 are essential for hydrolysis of crystalline chitin (2003), Biochem. J., 376, 237-244.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Escherichia coli Niallia circulans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 W164A decreased hydrolysing activity against highly crystalline beta-chitin and colloidal chitin, but enhanced activities against soluble substrates Niallia circulans
3.2.1.14 W164A/W285A drastically decreased activity against all substrates Niallia circulans
3.2.1.14 W285A decreased hydrolysing activity against highly crystalline beta-chitin and colloidal chitin, but enhanced activities against soluble substrates Niallia circulans
3.2.1.14 W433A drastically decreased activity against all substrates Niallia circulans
3.2.1.14 W53A decreased hydrolysing activity against highly crystalline beta-chitin Niallia circulans
3.2.1.14 Y279F drastically decreased activity against all substrates Niallia circulans
3.2.1.14 Y56A decreased hydrolysing activity against highly crystalline beta-chitin Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.0005
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, Y279F Niallia circulans
3.2.1.14 0.002
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, wild-type Niallia circulans
3.2.1.14 0.003
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, W433A Niallia circulans
3.2.1.14 0.081
-
 reduced chitopentaose 37°C, pH 6, wild-type Niallia circulans
3.2.1.14 0.177
-
 reduced chitopentaose 37°C, pH 6, W285A Niallia circulans
3.2.1.14 0.206
-
 reduced chitopentaose 37°C, pH 6, W164A Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Niallia circulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl-tri-N-acetylchitotriose + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.14 beta-chitin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.14 carboxymethyl chitin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.14 reduced chitopentaose + H2O
-
 Niallia circulans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiA1
-
 Niallia circulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 0.005
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, W433A Niallia circulans
3.2.1.14 0.14
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, Y279F Niallia circulans
3.2.1.14 9.55
-
 carboxymethyl chitin 37°C, pH 6, wild-type Niallia circulans
3.2.1.14 22.4
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, wild-type Niallia circulans
3.2.1.14 24.1
-
 carboxymethyl chitin 37°C, pH 6, W164A Niallia circulans
3.2.1.14 25.2
-
 carboxymethyl chitin 37°C, pH 6, W285A Niallia circulans
3.2.1.14 26.9
-
 reduced chitopentaose 37°C, pH 6, wild-type Niallia circulans
3.2.1.14 35.6
-
 reduced chitopentaose 37°C, pH 6, W164A Niallia circulans
3.2.1.14 45.1
-
 reduced chitopentaose 37°C, pH 6, W285A Niallia circulans