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Literature summary extracted from

  • Berrin, J.G.; Czjzek, M.; Kroon, P.A.; McLauchlan, W.R.; Puigserver, A.; Williamson, G.; Juge, N.
    Substrate (aglycone) specificity of human cytosolic beta-glucosidase (2003), Biochem. J., 373, 41-48.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Pichia pastoris Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.21 F225S 0.65% of wild-type specific activity with p-nitrophenyl-beta-D-glucopyranoside Homo sapiens
3.2.1.21 V168Y 12% of wild-type specific activity with p-nitrophenyl-beta-D-glucopyranoside Homo sapiens
3.2.1.21 Y308A 0.3% of wild-type specific activity with p-nitrophenyl-beta-D-glucopyranoside Homo sapiens
3.2.1.21 Y308F 6% of wild-type specific activity with p-nitrophenyl-beta-D-glucopyranoside Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.37
-
 p-nitrophenyl-beta-D-fucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 0.57
-
 p-nitrophenyl-beta-D-arabinopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 1.76
-
 p-nitrophenyl-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 3.14
-
 p-nitrophenyl-beta-D-galactopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 10
-
 luteolin-4'-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 31.8
-
 quercetin-4'-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 42.2
-
 quercetin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 50
-
 luteolin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 118
-
 daidzein 7-O-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 253
-
 eriodictyol-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 432
-
 naringenin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 53000
-
 1 * 53000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 apigenin-7-beta-glucopyranoside + H2O Homo sapiens
-
 apigenin + beta-D-glucose
-
 ?
3.2.1.21 daidzein 7-O-beta-D-glucopyranoside + H2O Homo sapiens
-
 daidzein + beta-D-glucose
-
 ?
3.2.1.21 eriodicytol-7-beta-D-glucopyranoside + H2O Homo sapiens
-
 eriodicytol + beta-D-glucose
-
 ?
3.2.1.21 luteolin-4'-beta-glucopyranoside + H2O Homo sapiens
-
 luteolin + beta-D-glucose
-
 ?
3.2.1.21 luteolin-7-beta-glucopyranoside + H2O Homo sapiens
-
 luteolin + beta-D-glucose
-
 ?
3.2.1.21 naringenin-7-beta-glucopyranoside + H2O Homo sapiens
-
 naringenin + beta-D-glucose
-
 ?
3.2.1.21 quercetin 4'-O-beta-D-glucopyranoside + H2O Homo sapiens
-
 quercetin + beta-D-glucose
-
 ?
3.2.1.21 quercetin 7-O-beta-D-glucopyranoside + H2O Homo sapiens
-
 quercetin + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 recombinant hCBG Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 intestine
-
 Homo sapiens
-
3.2.1.21 kidney
-
 Homo sapiens
-
3.2.1.21 liver
-
 Homo sapiens
-
3.2.1.21 spleen
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 apigenin-7-beta-glucopyranoside + H2O
-
 Homo sapiens apigenin + beta-D-glucose
-
 ?
3.2.1.21 daidzein 7-O-beta-D-glucopyranoside + H2O
-
 Homo sapiens daidzein + beta-D-glucose
-
 ?
3.2.1.21 eriodicytol-7-beta-D-glucopyranoside + H2O
-
 Homo sapiens eriodicytol + beta-D-glucose
-
 ?
3.2.1.21 luteolin-4'-beta-glucopyranoside + H2O
-
 Homo sapiens luteolin + beta-D-glucose
-
 ?
3.2.1.21 luteolin-7-beta-glucopyranoside + H2O
-
 Homo sapiens luteolin + beta-D-glucose
-
 ?
3.2.1.21 naringenin-7-beta-glucopyranoside + H2O
-
 Homo sapiens naringenin + beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-arabinopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-arabinose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-fucopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 quercetin 4'-O-beta-D-glucopyranoside + H2O
-
 Homo sapiens quercetin + beta-D-glucose
-
 ?
3.2.1.21 quercetin 7-O-beta-D-glucopyranoside + H2O
-
 Homo sapiens quercetin + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 53000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 hCBG
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 37
-
 20% loss of activity after 24 h Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 0.69
-
 quercetin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 1.08
-
 quercetin-4'-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 1.17
-
 luteolin-4'-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 1.26
-
 eriodictyol-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 2.6
-
 naringenin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 3.05
-
 luteolin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 3.55
-
 daidzein 7-O-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 6
-
 p-nitrophenyl-beta-D-arabinopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 10.7
-
 p-nitrophenyl-beta-D-fucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 12.1
-
 p-nitrophenyl-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.2.1.21 17.6
-
 p-nitrophenyl-beta-D-galactopyranoside 50°C, pH 6.5 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.5
-
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Homo sapiens
-
-
 4.9