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Literature summary extracted from

  • Kusmierczyk, A.R.; Martin, J.
    Nucleotide-dependent protein folding in the type II chaperonin from the mesophilic archaeon Methanococcus maripaludis (2003), Biochem. J., 371, 669-673.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.7 expression in Escherichia coli Methanococcus maripaludis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.7 900000
-
 gel filtration, recombinant Mm-cpn Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.7 ATP + H2O + a folded polypeptide Methanococcus maripaludis
-
 ADP + phosphate + an unfolded polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.7 Methanococcus maripaludis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.7 recombinant Mm-cpn, Mono Q, Superdex S-300 Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.7 ATP + H2O + a folded polypeptide
-
 Methanococcus maripaludis ADP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 ATP + H2O + a folded polypeptide ATP hydrolysis is not essentially required for protein folding, i.e. chaperone activity of Mm-cpn Methanococcus maripaludis ADP + phosphate + an unfolded polypeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.7 Mm-cpn
-
 Methanococcus maripaludis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.1.7 50
-
-
 Methanococcus maripaludis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.6.1.7 30 65 approx. 65% of maximal activity at 30°C, approx. 15% of maximal activity at 65°C Methanococcus maripaludis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.6.1.7 60
-
 significant loss of activity Methanococcus maripaludis
5.6.1.7 70
-
 complete loss of activity Methanococcus maripaludis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6.1.7 0.99
-
 ATP 37°C Methanococcus maripaludis