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Literature summary extracted from

  • Steenbakkers, P.J.M.; Harhangi, H.R.; Bosscher, M.W.; van der Hooft, M.M.C.; Keltjens, J.T.; van der Drift, C.; Vogels, G.D.; Op den Camp, H.J.M.
    beta-Glucosidase in cellulosome of the anaerobic fungus Piromyces sp. strain E2 is a family 3 glycoside hydrolase (2003), Biochem. J., 370, 963-970.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Piromyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 beta-D-glucose 30 mM, 50% inhibition Piromyces sp.
3.2.1.21 D-glucono-1,5-lactone 0.1 mM, 50% inhibition Piromyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.6
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 6.0 Piromyces sp.
3.2.1.21 1.8
-
 cellobiose 40°C, pH 6.0 Piromyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 cellulosome
-
 Piromyces sp. 43263
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 91457
-
 x * 91457, deduced from nucleotide sequence Piromyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 beta-D-glucosyl-cellulose + H2O Piromyces sp.
-
 cellulose + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Piromyces sp. Q875K3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 recombinant Cel3A Piromyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 beta-D-glucosyl-cellulose + H2O
-
 Piromyces sp. cellulose + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Piromyces sp. 2 beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-cellobiose + H2O
-
 Piromyces sp. p-nitrophenyl-beta-D-glucopyranoside + beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Piromyces sp. p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-xylopyranoside + H2O 1% of activity with p-nitrophenyl-beta-D-glucopyranoside Piromyces sp. p-nitrophenol + beta-D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 ? x * 91457, deduced from nucleotide sequence Piromyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Cel3A
-
 Piromyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 40
-
-
 Piromyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
-
 Piromyces sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Piromyces sp. deduced from nucleotide sequence
-
 4.9