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Literature summary extracted from

  • Marrakchi, H.; Dewolf, W.E., Jr.; Quinn, C.; West, J.; Polizzi, B.J.; So, C.Y.; Holmes, D.J.; Reed, S.L.; Heath, R.J.; Payne, D.J.; Rock, C.O.; Wallis, N.G.
    Characterization of Streptococcus pneumoniae enoyl-(acyl-carrier protein) reductase (FabK) (2003), Biochem. J., 370, 1055-1062.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 expression in Escherichia coli Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 0.009
-
 trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5, in the presence of 100 mM NH4+ Streptococcus pneumoniae
1.3.1.9 0.023
-
 NADH 30°C, pH 6.5, in the presence of 100 mM NH4+ Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.9 Cs+ activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ Streptococcus pneumoniae
1.3.1.9 K+ activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ Streptococcus pneumoniae
1.3.1.9 Na+ activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ Streptococcus pneumoniae
1.3.1.9 NH4+ 15fold activation at 100 mM Streptococcus pneumoniae
1.3.1.9 Rb+ activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 recombinant FabK Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 trans-but-2-enoyl-[acyl-carrier protein] + NADH highly selective for NADH Streptococcus pneumoniae butanoyl-[acyl-carrrier-protein] + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 FabK
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.9 0.016
-
 trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5, in the absence of 100 mM NH4+ Streptococcus pneumoniae
1.3.1.9 70
-
 trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5, in the presence of 100 mM NH4+ Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 FMN
-
 Streptococcus pneumoniae
1.3.1.9 NADH
-
 Streptococcus pneumoniae