Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zyryanov, A.B.; Shestakov, A.S.; Lahti, R.; Baykov, A.A.
    Mechanism by which metal cofactors control substrate specificity in pyrophosphatase (2002), Biochem. J., 367, 901-906.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.1 K193R decrease in activity Saccharomyces cerevisiae
3.6.1.1 K56R large decrease in activity Saccharomyces cerevisiae
3.6.1.1 R78K increased activity Saccharomyces cerevisiae
3.6.1.1 Y93F large decrease in activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 ATP competitive Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Co2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Saccharomyces cerevisiae
3.6.1.1 Mg2+ required Escherichia coli
3.6.1.1 Mg2+ required Rattus norvegicus
3.6.1.1 Mg2+ required Saccharomyces cerevisiae
3.6.1.1 Mg2+ required Streptococcus mutans
3.6.1.1 Mn2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Escherichia coli
3.6.1.1 Mn2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Rattus norvegicus
3.6.1.1 Mn2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Saccharomyces cerevisiae
3.6.1.1 Zn2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Escherichia coli important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Rattus norvegicus important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Saccharomyces cerevisiae important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Streptococcus mutans important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Escherichia coli MRE 600 important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Escherichia coli
-
-
-
3.6.1.1 Escherichia coli MRE 600
-
-
-
3.6.1.1 Rattus norvegicus
-
-
-
3.6.1.1 Saccharomyces cerevisiae
-
-
-
3.6.1.1 Streptococcus mutans
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.1 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 ATP + H2O reaction only in the presence of Mn2+ Escherichia coli ?
-
 ?
3.6.1.1 ATP + H2O reaction only in the presence of Mn2+ Rattus norvegicus ?
-
 ?
3.6.1.1 ATP + H2O reaction only in the presence of Mn2+ or Zn2+ Saccharomyces cerevisiae ?
-
 ?
3.6.1.1 ATP + H2O reaction only in the presence of Mn2+ Escherichia coli MRE 600 ?
-
 ?
3.6.1.1 diphosphate + H2O
-
 Escherichia coli 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Rattus norvegicus 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Saccharomyces cerevisiae 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Streptococcus mutans 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Escherichia coli 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Rattus norvegicus 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Saccharomyces cerevisiae 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Streptococcus mutans 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Escherichia coli MRE 600 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Escherichia coli MRE 600 2 phosphate
-
 ?
3.6.1.1 additional information no hydrolysis of ATP and traces of activity with tripolyphosphate in the presence of Mg2+ Saccharomyces cerevisiae ?
-
 ?
3.6.1.1 additional information no hydrolysis of ATP in the presence of Mg2+ Escherichia coli ?
-
 ?
3.6.1.1 additional information no hydrolysis of ATP in the presence of Mg2+ Rattus norvegicus ?
-
 ?
3.6.1.1 additional information no hydrolysis of ATP in the presence of Mg2+ Streptococcus mutans ?
-
 ?
3.6.1.1 additional information no hydrolysis of ATP in the presence of Mg2+ Escherichia coli MRE 600 ?
-
 ?
3.6.1.1 tripolyphosphate + H2O reaction only in the presence of Mn2+ or Zn2+ Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.1 0.005
-
 ATP less than 0.005/sec, pH 7.2, 25°C, in the presence of Mg2+ Saccharomyces cerevisiae
3.6.1.1 0.05
-
 tripolyphosphate pH 7.2, 25°C, in the presence of Mg2+ Saccharomyces cerevisiae
3.6.1.1 260
-
 diphosphate pH 7.2, 25°C, in the presence of Mg2+ Saccharomyces cerevisiae