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Literature summary extracted from

  • Fernandez, C.; Suarez, Y.; Ferruelo, A.J.; Gomez-Coronado, D.; Lasuncion, M.A.
    Inhibition of cholesterol biosynthesis by DELTA22-unsaturated phytosterols via competitive inhibition of sterol DELTA24-reductase in mammalian cells (2002), Biochem. J., 366, 109-119.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.72 5,22-cholestedien-3beta-ol desmosterol isomer, competitive inhibition Homo sapiens
1.3.1.72 5,22-cholestedien-3beta-ol desmosterol isomer, competitive inhibition Rattus norvegicus
1.3.1.72 brassicasterol DELTA22-unsaturated phytosterol, competitive inhibition Homo sapiens
1.3.1.72 brassicasterol DELTA22-unsaturated phytosterol, competitive inhibition Rattus norvegicus
1.3.1.72 ergosterol DELTA22-unsaturated phytosterol, competitive inhibition Homo sapiens
1.3.1.72 ergosterol DELTA22-unsaturated phytosterol, competitive inhibition Rattus norvegicus
1.3.1.72 additional information inhibition mechanism of DELTA22-unsaturated phytosterols Homo sapiens
1.3.1.72 additional information inhibition mechanism of DELTA22-unsaturated phytosterols Rattus norvegicus
1.3.1.72 stigmasterol DELTA22-unsaturated phytosterol, competitive inhibition Homo sapiens
1.3.1.72 stigmasterol DELTA22-unsaturated phytosterol, competitive inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.72 26.3
-
 desmosterol pH 7.6, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.72 membrane bound Homo sapiens 16020
-
1.3.1.72 membrane bound Rattus norvegicus 16020
-
1.3.1.72 microsome
-
 Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.72 5alpha-cholesta-7,24-dien-3beta-ol + NADPH Homo sapiens step in the biosynthesis of cholesterol from lanosterol 5alpha-cholest-7-en-3beta-ol + NADP+
-
 ?
1.3.1.72 5alpha-cholesta-7,24-dien-3beta-ol + NADPH Rattus norvegicus step in the biosynthesis of cholesterol from lanosterol 5alpha-cholest-7-en-3beta-ol + NADP+
-
 ?
1.3.1.72 cholesta-5,7,24-trien-3beta-ol + NADPH Homo sapiens step in the biosynthesis of cholesterol from lanosterol cholest-5,7-dien-3beta-ol + NADP+
-
 ?
1.3.1.72 cholesta-5,7,24-trien-3beta-ol + NADPH Rattus norvegicus step in the biosynthesis of cholesterol from lanosterol cholest-5,7-dien-3beta-ol + NADP+
-
 ?
1.3.1.72 desmosterol + NADPH Homo sapiens step in the biosynthesis of cholesterol from lanosterol cholesterol + NADP+
-
 ?
1.3.1.72 desmosterol + NADPH Rattus norvegicus step in the biosynthesis of cholesterol from lanosterol cholesterol + NADP+
-
 ?
1.3.1.72 lanosterol + NADPH Homo sapiens step in the biosynthesis of cholesterol from lanosterol 24-dihydrolanosterol + NADP+
-
 ?
1.3.1.72 lanosterol + NADPH Rattus norvegicus step in the biosynthesis of cholesterol from lanosterol 24-dihydrolanosterol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.72 Homo sapiens
-
-
-
1.3.1.72 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.72 Caco-2 cell intestinal mucosa cells Homo sapiens
-
1.3.1.72 HL-60 cell promyelocytic cells Homo sapiens
-
1.3.1.72 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.72 5alpha-cholesta-7,24-dien-3beta-ol + NADPH best substrate Homo sapiens 5alpha-cholest-7-en-3beta-ol + NADP+ i.e.lathosterol ?
1.3.1.72 5alpha-cholesta-7,24-dien-3beta-ol + NADPH best substrate Rattus norvegicus 5alpha-cholest-7-en-3beta-ol + NADP+ i.e.lathosterol ?
1.3.1.72 5alpha-cholesta-7,24-dien-3beta-ol + NADPH step in the biosynthesis of cholesterol from lanosterol Homo sapiens 5alpha-cholest-7-en-3beta-ol + NADP+
-
 ?
1.3.1.72 5alpha-cholesta-7,24-dien-3beta-ol + NADPH step in the biosynthesis of cholesterol from lanosterol Rattus norvegicus 5alpha-cholest-7-en-3beta-ol + NADP+
-
 ?
1.3.1.72 cholesta-5,7,24-trien-3beta-ol + NADPH
-
 Homo sapiens cholest-5,7-dien-3beta-ol + NADP+
-
 ?
1.3.1.72 cholesta-5,7,24-trien-3beta-ol + NADPH
-
 Rattus norvegicus cholest-5,7-dien-3beta-ol + NADP+
-
 ?
1.3.1.72 cholesta-5,7,24-trien-3beta-ol + NADPH step in the biosynthesis of cholesterol from lanosterol Homo sapiens cholest-5,7-dien-3beta-ol + NADP+
-
 ?
1.3.1.72 cholesta-5,7,24-trien-3beta-ol + NADPH step in the biosynthesis of cholesterol from lanosterol Rattus norvegicus cholest-5,7-dien-3beta-ol + NADP+
-
 ?
1.3.1.72 desmosterol + NADPH
-
 Homo sapiens cholesterol + NADP+
-
 ?
1.3.1.72 desmosterol + NADPH
-
 Rattus norvegicus cholesterol + NADP+
-
 ?
1.3.1.72 desmosterol + NADPH step in the biosynthesis of cholesterol from lanosterol Homo sapiens cholesterol + NADP+
-
 ?
1.3.1.72 desmosterol + NADPH step in the biosynthesis of cholesterol from lanosterol Rattus norvegicus cholesterol + NADP+
-
 ?
1.3.1.72 lanosterol + NADPH
-
 Homo sapiens 24-dihydrolanosterol + NADP+
-
 ?
1.3.1.72 lanosterol + NADPH
-
 Rattus norvegicus 24-dihydrolanosterol + NADP+
-
 ?
1.3.1.72 lanosterol + NADPH step in the biosynthesis of cholesterol from lanosterol Homo sapiens 24-dihydrolanosterol + NADP+
-
 ?
1.3.1.72 lanosterol + NADPH step in the biosynthesis of cholesterol from lanosterol Rattus norvegicus 24-dihydrolanosterol + NADP+
-
 ?
1.3.1.72 zymosterol + NADPH
-
 Homo sapiens 5alpha-cholesta-8-en-3beta-ol + NADP+
-
 ?
1.3.1.72 zymosterol + NADPH
-
 Rattus norvegicus 5alpha-cholesta-8-en-3beta-ol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.72 desmosterol reductase
-
 Homo sapiens
1.3.1.72 desmosterol reductase
-
 Rattus norvegicus
1.3.1.72 lanosterol reductase
-
 Homo sapiens
1.3.1.72 lanosterol reductase
-
 Rattus norvegicus
1.3.1.72 sterol 24DELTA-reductase
-
 Homo sapiens
1.3.1.72 sterol 24DELTA-reductase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.72 37
-
 assay at Homo sapiens
1.3.1.72 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.72 7.6
-
 assay at Homo sapiens
1.3.1.72 7.6
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.72 NADPH
-
 Homo sapiens
1.3.1.72 NADPH
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.72 0.0033
-
 5,22-cholestedien-3beta-ol pH 7.6, 37°C Rattus norvegicus
1.3.1.72 0.037
-
 ergosterol pH 7.6, 37°C Rattus norvegicus
1.3.1.72 0.041
-
 stigmasterol pH 7.6, 37°C Rattus norvegicus
1.3.1.72 0.043
-
 brassicasterol pH 7.6, 37°C Rattus norvegicus