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Literature summary extracted from

  • Becker, D.; Braet, C.; Brumer, H.3rd.; Claeyssens, M.; Divne, C.; Fagerstrom, B.R.; Harris, M.; Jones, T.A.; Kleywegt, G.J.; Koivula, A.; Mahdi, S.; Piens, K.; Sinnott, M.L.; Stahlberg, J.; Teeri, T.T.; Underwood, M.; Wohlfahrt, G.
    Engineering of a glycosidase family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/A224H/L225V/T226A/D262G mutant (2001), Biochem. J., 356, 19-30.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176
-
 Trichoderma reesei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.176 hanging drop vapor diffusion method Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 E223S/A224H/L225V/T226A/D262G mutant has a more alkaline pH optimum Trichoderma reesei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 cellobiose competitive with wild-type enzyme, mixed inhibition with mutant enzyme Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 11
-
 3,4-dinitrophenyl-cellobioside 25°C, pH 7, wild-type Trichoderma reesei
3.2.1.176 14
-
 3,4-dinitrophenyl-cellobioside 25°C, pH 5, wild-type Trichoderma reesei
3.2.1.176 48
-
 3,4-dinitrophenyl-cellobioside 25°C, pH 5, E223S/A224H/L225V/T226A/D262G mutant Trichoderma reesei
3.2.1.176 161
-
 3,4-dinitrophenyl-cellobioside 25°C, pH 7, E223S/A224H/L225V/T226A/D262G mutant Trichoderma reesei
3.2.1.176 380
-
 3,4-dinitrophenyl-lactoside 25°C, pH 5, wild-type Trichoderma reesei
3.2.1.176 543
-
 3,4-dinitrophenyl-lactoside 25°C, pH 5, E223S/A224H/L225V/T226A/D262G mutant Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Trichoderma reesei P62694
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 2-chloro-4-nitrophenyl lactoside + H2O
-
 Trichoderma reesei 2-chloro-4-nitrophenol + ?
-
 ?
3.2.1.176 3,4-dinitrophenyl lactoside + H2O
-
 Trichoderma reesei 3,4-dinitrophenol + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 50
-
 enzyme retains full activity between pH 4 and 6, but is rapidly inactivated at lower pH values Trichoderma reesei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.176 2 7 enzyme is stable at pH 3-7 at 37°C, but loses 90% of activity at pH 2 after 20 min Trichoderma reesei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.176 0.02
-
 cellobiose 25°C, wild-type enzyme Trichoderma reesei
3.2.1.176 0.755
-
 cellobiose 25°C, E223S/A224H/L225V/T226A/D262G mutant Trichoderma reesei