Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • George, R.; Kelly, S.M.; Price, N.C.; Erbse, A.; Fisher, M.; Lund, P.A.
    Three GroEL homologues from Rhizobium leguminosarum have distinct in vitro properties (2004), Biochem. Biophys. Res. Commun., 324, 822-828.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.7 expression in Escherichia coli Escherichia coli
5.6.1.7 expression in Escherichia coli Rhizobium leguminosarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.7 ATP + H2O + a folded polypeptide Escherichia coli
-
 ADP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 ATP + H2O + a folded polypeptide Rhizobium leguminosarum
-
 ADP + phosphate + an unfolded polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.7 Escherichia coli
-
-
-
5.6.1.7 Rhizobium leguminosarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.7 recombinant Cpn60-1 Rhizobium leguminosarum
5.6.1.7 recombinant Cpn60-2 Rhizobium leguminosarum
5.6.1.7 recombinant Cpn60-3 Rhizobium leguminosarum
5.6.1.7 recombinant GroEL Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.7 ATP + H2O + a folded polypeptide
-
 Escherichia coli ADP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 ATP + H2O + a folded polypeptide
-
 Rhizobium leguminosarum ADP + phosphate + an unfolded polypeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.7 Cpn60-1
-
 Rhizobium leguminosarum
5.6.1.7 Cpn60-2
-
 Rhizobium leguminosarum
5.6.1.7 Cpn60-3
-
 Rhizobium leguminosarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.1.7 60
-
-
 Escherichia coli
5.6.1.7 60
-
-
 Rhizobium leguminosarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.6.1.7 25 70 approx. 15% of maximal activity at 40°C, approx. 20% of maximal activity at 68°C Escherichia coli
5.6.1.7 25 70 approx. 20% of maximal activity at 40°C and 70°C, respectively Rhizobium leguminosarum
5.6.1.7 25 70 approx. 20% of maximal activity at 40°C, approx. 20% of maximal activity at 68°C Rhizobium leguminosarum
5.6.1.7 25 70 approx. 60% of maximal activity at 40°C, approx. 20% of maximal activity at 68°C Rhizobium leguminosarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6.1.7 0.003
-
 ATP 20°C Rhizobium leguminosarum
5.6.1.7 0.009
-
 ATP 20°C Rhizobium leguminosarum
5.6.1.7 0.014
-
 ATP 20°C Rhizobium leguminosarum
5.6.1.7 0.025
-
 ATP 20°C Escherichia coli