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Literature summary extracted from

  • Okuda, J.; Sode, K.
    PQQ glucose dehydrogenase with novel electron transfer ability (2004), Biochem. Biophys. Res. Commun., 314, 793-797.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.5.2 biotechnology engineering of the soluble enzyme GDH-B to enable the electron transfer to the electrode in absence of artificial electron mediator by mimicking the domain structure of the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni, which is composed of a PQQ-containing catalytic domain and a cytochrome c domain Acinetobacter calcoaceticus
1.1.5.2 diagnostics enzyme is industrially used as glucose sensor with high catalytic activity and insensitivity to oxygen Acinetobacter calcoaceticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 gene gdhB, expression of the enzyme GDH-B as fusion protein C-terminally fused to cytochrome c domain from Comamonas testosteroni in Escherichia coli DH5alpha Acinetobacter calcoaceticus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 additional information the recombinant cytochrome c-fusion protein shows a highly increased sensitivity when immobilized to the electrode as D-glucose sensor compared to the wild-type enzyme, overview Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 2 3 D-glucose recombinant cytochrome c-fusion protein, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 15
-
 maltose recombinant cytochrome c-fusion protein, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 19
-
 lactose recombinant cytochrome c-fusion protein, pH 7.0 Acinetobacter calcoaceticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 soluble
-
 Acinetobacter calcoaceticus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.2 Ca2+ required for structure stabilization of the holoform Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 65000
-
 x * 65000, recombinant cytochrome c-fusion protein, SDS-PAGE Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone Acinetobacter calcoaceticus
-
 D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone Acinetobacter calcoaceticus LMD79.41
-
 D-glucono-1,5-lactone + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus
-
-
-
1.1.5.2 Acinetobacter calcoaceticus LMD79.41
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2 recombinant cytochrome c fusion protein from Escherichia coli Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-glucose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus LMD79.41 D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone
-
 Acinetobacter calcoaceticus LMD79.41 D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 lactose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 lactose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus LMD79.41 ?
-
 ?
1.1.5.2 maltose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 maltose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus LMD79.41 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 ? x * 65000, recombinant cytochrome c-fusion protein, SDS-PAGE Acinetobacter calcoaceticus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 GDH-B
-
 Acinetobacter calcoaceticus
1.1.5.2 PQQ glucose dehydrogenase
-
 Acinetobacter calcoaceticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.2 3178
-
 D-glucose recombinant cytochrome c-fusion protein, pH 7.0 Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 7
-
 assay at Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone i.e. PQQ, dependent on Acinetobacter calcoaceticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.5.2 Acinetobacter calcoaceticus calculated, recombinant cytochrome c-fusion protein 4.7 4.6
1.1.5.2 Acinetobacter calcoaceticus calculated, wild-type GDH-B 4.7 4.6