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Literature summary extracted from

  • Lee, M.D.; Antczak, C.; Li, Y.; Sirotnak, F.M.; Bornmann, W.G.; Scheinberg, D.A.
    A new human peptide deformylase inhibitable by actinonin (2003), Biochem. Biophys. Res. Commun., 312, 309-315.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.88 mutant lacking the first 63 amino acids is expressed in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.88 actinonin competitive, IC50: 43 nM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.88 0.047
-
 formyl-Met-Leu-p-nitroanilide
-
 Homo sapiens
3.5.1.88 11
-
 formyl-Met-Ala-Ser
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.88 Co2+ the Co2+-containing enzyme form is active Homo sapiens
3.5.1.88 additional information Ni2+ and Zn2+-containing enzyme forms are inactive Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.88
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.88 HL-60 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 formyl-Met-Ala-Ser + H2O
-
 Homo sapiens formate + Met-Ala-Ser
-
 ?
3.5.1.88 formyl-Met-Leu-p-nitroanilide + H2O
-
 Homo sapiens formate + Met-Leu-p-nitroanilide
-
 ?
3.5.1.88 additional information no detectable activity with formyl-Met-Leu-Phe and formyl-Met-Leu-Pro Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.88 25 50
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.88 0.00046
-
 formyl-Met-Leu-p-nitroanilide
-
 Homo sapiens
3.5.1.88 0.0081
-
 formyl-Met-Ala-Ser
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.88 6 7.2
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.88 0.000043
-
 competitive, IC50: 43 nM Homo sapiens actinonin