Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Suzuki, A.; Yao, M.; Tanaka, I.; Numata, T.; Kikukawa, S.; Yamasaki, N.; Kimura, M.
    Crystal structures of the ribonuclease MC1 from bitter gourd seeds, complexed with 2'-UMP or 3'-UMP, reveal structural basis for uridine specificity (2000), Biochem. Biophys. Res. Commun., 275, 572-576.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.19 expression of mutant enzymes in Escherichia coli BL21(DE3) Momordica charantia

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.19 19 mg/ml wild-type enzyme in complex with 2'-UMP and 3'-UMP, hanging drop vapour diffusion method, reservoir solution contains 0.2 M sodium acetate, 0.1 M sodium cacodylate, pH 6.7, 21% PEG 8000, 10 mM 2'-UMP or 3'-UMP, 4 days, X-ray diffraction structure determination and analysis at 1.48 and 1.77 A resolution, respectively Momordica charantia

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.19 L73A site-directed mutagenesis, slightly increased catalytic efficiency and 14.5fold decreased binding affinity compared to the wild-type enzyme Momordica charantia
4.6.1.19 N71T site-directed mutagenesis, 2.3fold decreased catalytic efficiency and 7.0fold decreased binding affinity compared to the wild-type enzyme Momordica charantia
4.6.1.19 R74S site-directed mutagenesis, decreased catalytic efficiency and 0.8fold increased binding affinity compared to the wild-type enzyme Momordica charantia
4.6.1.19 V72L site-directed mutagenesis, catalytic efficiency and binding affinity similar to the wild-type enzyme Momordica charantia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.19 1.1
-
 CpU mutant R74S, pH 6.5, 37°C Momordica charantia
4.6.1.19 1.38
-
 CpU wild-type enzyme, pH 6.5, 37°C Momordica charantia
4.6.1.19 1.57
-
 CpU mutant V72L, pH 6.5, 37°C Momordica charantia
4.6.1.19 9.63
-
 CpU mutant N71T, pH 6.5, 37°C Momordica charantia
4.6.1.19 20
-
 CpU mutant L73A, pH 6.5, 37°C Momordica charantia

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.19 Momordica charantia
-
 bitter gourd
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.6.1.19 RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] substrate recognition, binding and specificity, Asn71 and Leu73 are involved Momordica charantia
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.19 seed
-
 Momordica charantia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.19 CpU + H2O
-
 Momordica charantia ?
-
 ?
4.6.1.19 additional information enzyme preferentially cleaves the 5'-side of uridine, Asn71 and Leu73 are involved in substrate specificity Momordica charantia ?
-
 ?
4.6.1.19 RNA + H2O
-
 Momordica charantia ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.19 More enzyme belongs to the ribonuclease T2 family Momordica charantia
4.6.1.19 ribonuclease MC1
-
 Momordica charantia
4.6.1.19 RNase MC1
-
 Momordica charantia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.19 37
-
 assay at Momordica charantia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6.1.19 5.37
-
 CpU mutant N71T, pH 6.5, 37°C Momordica charantia
4.6.1.19 7.38
-
 CpU mutant R74S, pH 6.5, 37°C Momordica charantia
4.6.1.19 12.3
-
 CpU mutant V72L, pH 6.5, 37°C Momordica charantia
4.6.1.19 12.42
-
 CpU wild-type enzyme, pH 6.5, 37°C Momordica charantia
4.6.1.19 13.1
-
 CpU mutant L73A, pH 6.5, 37°C Momordica charantia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.19 6.5
-
 assay at Momordica charantia