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Literature summary extracted from

  • Blanchard, C.Z.; Amspacher, D.; Strongin, R.; Waldrop, G.L.
    Inhibition of biotin carboxylase by a reaction intermediate analog: implications for the kinetic mechanism (1999), Biochem. Biophys. Res. Commun., 266, 466-471.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.14 biotin above 300 mM, noncompetitive substrate inhibitor Escherichia coli
6.3.4.14 additional information no substrate inhibition by ATP Escherichia coli
6.3.4.14 Phosphonoacetate competitive inhibition versus ATP, noncompetitive versus bicarbonate Escherichia coli
6.3.4.14 phosphonoacetate linked to the 1'-nitrogen of biotin reaction intermediate analog, modest inhibition, competitive versus ATP, noncompetitive versus biotin Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.14 additional information
-
 additional information ordered kinetic mechanism Escherichia coli
6.3.4.14 134
-
 biotin
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.14 Mg2+ requirement Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3- Escherichia coli long-chain fatty acid synthesis, in vivo biotin is covalently attached to the biotin-carboxyl-carrier protein ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.14
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.14 ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine kinetic mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ATP + biotin + HCO3- biotin carboxylase component of the multienzyme complex acetyl-CoA carboxylase, also utilizes free biotin as substrate Escherichia coli ADP + phosphate + carboxybiotin
-
 ?
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3- long-chain fatty acid synthesis, in vivo biotin is covalently attached to the biotin-carboxyl-carrier protein Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3- biotin carboxylase component of the multienzyme complex acetyl-CoA carboxylase, catalyzes the first half-reaction of the reaction catalyzed by acetyl-CoA carboxylase, the ATP-dependent carboxylation of biotin, ordered addition of substrates with ATP binding first followed by bicarbonate and then biotin Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.14 ATP
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.4.14 7
-
 Phosphonoacetate
-
 Escherichia coli
6.3.4.14 8
-
 phosphonoacetate linked to the 1'-nitrogen of biotin
-
 Escherichia coli