EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.5.2 | biotechnology | bioengineering of water-soluble isozyme PQQGDH-B production at industrial level | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.5.2 | expression of wild-type and mutant isozymes PQQGDH-B | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.5.2 | D448N | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | D456N | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | D457N | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | N452D | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | N452H | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | N452I | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | N452K | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | N452T | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows narrowed substrate specificity, but unaltered catalytic efficiency, thermal stability, and EDTA tolerance compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | N462D | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | N462H | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | N462K | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.5.2 | N462Y | site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B | Escherichia coli |
1.1.99.35 | D448N | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 72%, D-allose 39%, D-galactose 14%, D-maltose 36%, D-lactose 48%, respectively | Acinetobacter calcoaceticus |
1.1.99.35 | D456N | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 78%, D-allose 43%, D-galactose 16%, D-maltose 41%, D-lactose 59%, respectively | Acinetobacter calcoaceticus |
1.1.99.35 | DD457N | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 80%, D-allose 46%, D-galactose 11%, D-maltose 32%, D-lactose 43%, respectively | Acinetobacter calcoaceticus |
1.1.99.35 | N452T | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 59%, D-allose 33%, D-galactose 5%, D-maltose 30%, D-lactose 31%, respectively | Acinetobacter calcoaceticus |
1.1.99.35 | N462H | substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 53%, D-allose 32%, D-galactose 2%, D-maltose 25%, D-lactose 31%, respectively | Acinetobacter calcoaceticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.2 | 2.7 | - |
D-galactose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 3.7 | - |
D-galactose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 5.3 | - |
D-galactose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 12.3 | - |
D-glucose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 12.5 | - |
D-glucose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 16 | - |
maltose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 18 | - |
lactose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 18.9 | - |
lactose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 25 | - |
D-glucose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 26 | - |
maltose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 27.6 | - |
3-O-methyl-D-glucose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 28.7 | - |
3-O-methyl-D-glucose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 28.8 | - |
3-O-methyl-D-glucose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 32.5 | - |
allose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 33.6 | - |
lactose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 35.5 | - |
allose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 38.7 | - |
allose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 46.5 | - |
maltose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.99.35 | 2.7 | - |
D-galactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 3.7 | - |
D-galactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 5.3 | - |
D-galactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 12.3 | - |
D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 12.5 | - |
D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 16 | - |
D-maltose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 18 | - |
D-lactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 18.9 | - |
D-lactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 25 | - |
D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 26 | - |
D-maltose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 27.6 | - |
3-O-methyl-D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 28.7 | - |
3-O-methyl-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 28.8 | - |
3-O-methyl-D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 32.5 | - |
D-allose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 33.6 | - |
D-lactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 35.5 | - |
D-allose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 38.7 | - |
D-allose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 46.5 | - |
D-maltose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.2 | soluble | isoyzme PQQGDH-B | Escherichia coli | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.2 | Ca2+ | required | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.2 | Escherichia coli | - |
isozyme PQQGDH-B | - |
1.1.99.35 | Acinetobacter calcoaceticus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.5.2 | D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol | active site structure contains a loop 6BC region which does not directly interact with the substrates | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | additional information | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.2 | 3-O-methyl-D-glucose + ubiquinone | - |
Escherichia coli | 3-O-methyl-D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | allose + ubiquinone | - |
Escherichia coli | ? + ubiquinol | - |
? | |
1.1.5.2 | D-galactose + ubiquinone | low activity, recombinant wild-type and mutant isozymes PQQGDH-B | Escherichia coli | D-galactono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | D-glucose + ubiquinone | best substrate, recombinant wild-type and mutant isozymes PQQGDH-B | Escherichia coli | D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | lactose + ubiquinone | - |
Escherichia coli | ? + ubiquinol | - |
? | |
1.1.5.2 | maltose + ubiquinone | - |
Escherichia coli | ? + ubiquinol | - |
? | |
1.1.5.2 | additional information | substrate specificities of wild-type and mutant isozyme PQQGDH-B, overview | Escherichia coli | ? | - |
? | |
1.1.99.35 | 3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol | 81% of the activity with D-glucose | Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-allose + oxidized 2,6-dichlorophenolindophenol | 47% of the activity with D-glucose | Acinetobacter calcoaceticus | D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-galactose + oxidized 2,6-dichlorophenolindophenol | 11% of the activity with D-glucose | Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | lactose + oxidized 2,6-dichlorophenolindophenol | 61% of the activity with D-glucose | Acinetobacter calcoaceticus | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | maltose + oxidized 2,6-dichlorophenolindophenol | 61% of the activity with D-glucose | Acinetobacter calcoaceticus | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.5.2 | More | 3D-model prediction for wild-type and mutant isozyme PQQGDH-B | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.2 | PQQGDH-B | - |
Escherichia coli |
1.1.5.2 | water-soluble PQQ glucose dehydrogenase | - |
Escherichia coli |
1.1.99.35 | PQQ glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
1.1.99.35 | PQQGDH-B | - |
Acinetobacter calcoaceticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 25 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.2 | 69 | - |
D-galactose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 72 | - |
D-galactose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 232 | - |
D-galactose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 574 | - |
lactose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 588 | - |
maltose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 949 | - |
allose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 1002 | - |
maltose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 1035 | - |
allose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 1038 | - |
lactose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 1064 | - |
3-O-methyl-D-glucose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 1253 | - |
3-O-methyl-D-glucose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 1399 | - |
D-glucose | N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 1659 | - |
lactose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 1791 | - |
D-glucose | N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 1930 | - |
maltose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 2509 | - |
allose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 3011 | - |
3-O-methyl-D-glucose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.5.2 | 3860 | - |
D-glucose | wild-type isozyme PQQGDH-B, pH 7.0, 25°C | Escherichia coli | |
1.1.99.35 | 69 | - |
D-galactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 72 | - |
D-galactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 232 | - |
D-galactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 574 | - |
D-lactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 588 | - |
D-maltose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 949 | - |
D-allose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 1002 | - |
D-maltose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 1035 | - |
D-allose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 1038 | - |
D-lactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 1064 | - |
3-O-methyl-D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 1253 | - |
3-O-methyl-D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 1399 | - |
D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 1659 | - |
D-lactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 1791 | - |
D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 1930 | - |
D-maltose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 2509 | - |
D-allose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 3011 | - |
3-O-methyl-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 3860 | - |
D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 7 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.2 | pyrroloquinoline quinone | prosthetic group | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.35 | 22 | - |
D-maltose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 23 | - |
D-galactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 25 | - |
D-allose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 26 | - |
D-galactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 31 | - |
D-lactose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 32 | - |
D-allose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 32 | - |
D-lactose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 37 | - |
D-maltose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 37 | - |
3-O-methyl-D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 44 | - |
D-galactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 45 | - |
3-O-methyl-D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 71 | - |
D-allose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 74 | - |
D-maltose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 88 | - |
D-lactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 105 | - |
3-O-methyl-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 114 | - |
D-glucose | mutant N462H, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 143 | - |
D-glucose | mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1.1.99.35 | 154 | - |
D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus |