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Literature summary extracted from

  • Sode, K.; Igarashi, S.; Morimoto, A.; Yoshida, H.
    Construction of engineered water-soluble PQQ glucose dehydrogenase with improved substrate specificity (2002), Biocatal. Biotransform., 20, 405-412.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.5.2 biotechnology bioengineering of water-soluble isozyme PQQGDH-B production at industrial level Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 expression of wild-type and mutant isozymes PQQGDH-B Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 D448N site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 D456N site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 D457N site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 N452D site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 N452H site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 N452I site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 N452K site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 N452T site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows narrowed substrate specificity, but unaltered catalytic efficiency, thermal stability, and EDTA tolerance compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 N462D site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 N462H site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 N462K site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.5.2 N462Y site-directed mutagenesis, mutation in the active site loop 6BC region, mutant shows altered substrate specificity, but unaltered catalytic efficiency with D-glucose, compared to the wild-type isozyme PQQGDH-B Escherichia coli
1.1.99.35 D448N substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 72%, D-allose 39%, D-galactose 14%, D-maltose 36%, D-lactose 48%, respectively Acinetobacter calcoaceticus
1.1.99.35 D456N substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 78%, D-allose 43%, D-galactose 16%, D-maltose 41%, D-lactose 59%, respectively Acinetobacter calcoaceticus
1.1.99.35 DD457N substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 80%, D-allose 46%, D-galactose 11%, D-maltose 32%, D-lactose 43%, respectively Acinetobacter calcoaceticus
1.1.99.35 N452T substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 59%, D-allose 33%, D-galactose 5%, D-maltose 30%, D-lactose 31%, respectively Acinetobacter calcoaceticus
1.1.99.35 N462H substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 53%, D-allose 32%, D-galactose 2%, D-maltose 25%, D-lactose 31%, respectively Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 2.7
-
 D-galactose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 3.7
-
 D-galactose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 5.3
-
 D-galactose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 12.3
-
 D-glucose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 12.5
-
 D-glucose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 16
-
 maltose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 18
-
 lactose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 18.9
-
 lactose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 25
-
 D-glucose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 26
-
 maltose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 27.6
-
 3-O-methyl-D-glucose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 28.7
-
 3-O-methyl-D-glucose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 28.8
-
 3-O-methyl-D-glucose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 32.5
-
 allose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 33.6
-
 lactose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 35.5
-
 allose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 38.7
-
 allose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 46.5
-
 maltose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.99.35 2.7
-
 D-galactose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 3.7
-
 D-galactose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 5.3
-
 D-galactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 12.3
-
 D-glucose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 12.5
-
 D-glucose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 16
-
 D-maltose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 18
-
 D-lactose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 18.9
-
 D-lactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 25
-
 D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 26
-
 D-maltose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 27.6
-
 3-O-methyl-D-glucose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 28.7
-
 3-O-methyl-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 28.8
-
 3-O-methyl-D-glucose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 32.5
-
 D-allose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 33.6
-
 D-lactose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 35.5
-
 D-allose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 38.7
-
 D-allose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 46.5
-
 D-maltose mutant N452T, pH 7.0 Acinetobacter calcoaceticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 soluble isoyzme PQQGDH-B Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.2 Ca2+ required Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Escherichia coli
-
 isozyme PQQGDH-B
-
1.1.99.35 Acinetobacter calcoaceticus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.5.2 D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol active site structure contains a loop 6BC region which does not directly interact with the substrates Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 3-O-methyl-D-glucose + ubiquinone
-
 Escherichia coli 3-O-methyl-D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 allose + ubiquinone
-
 Escherichia coli ? + ubiquinol
-
 ?
1.1.5.2 D-galactose + ubiquinone low activity, recombinant wild-type and mutant isozymes PQQGDH-B Escherichia coli D-galactono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone best substrate, recombinant wild-type and mutant isozymes PQQGDH-B Escherichia coli D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 lactose + ubiquinone
-
 Escherichia coli ? + ubiquinol
-
 ?
1.1.5.2 maltose + ubiquinone
-
 Escherichia coli ? + ubiquinol
-
 ?
1.1.5.2 additional information substrate specificities of wild-type and mutant isozyme PQQGDH-B, overview Escherichia coli ?
-
 ?
1.1.99.35 3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol 81% of the activity with D-glucose Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-allose + oxidized 2,6-dichlorophenolindophenol 47% of the activity with D-glucose Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-galactose + oxidized 2,6-dichlorophenolindophenol 11% of the activity with D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-glucose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 lactose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 maltose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 More 3D-model prediction for wild-type and mutant isozyme PQQGDH-B Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 PQQGDH-B
-
 Escherichia coli
1.1.5.2 water-soluble PQQ glucose dehydrogenase
-
 Escherichia coli
1.1.99.35 PQQ glucose dehydrogenase
-
 Acinetobacter calcoaceticus
1.1.99.35 PQQGDH-B
-
 Acinetobacter calcoaceticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.2 25
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.2 69
-
 D-galactose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 72
-
 D-galactose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 232
-
 D-galactose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 574
-
 lactose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 588
-
 maltose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 949
-
 allose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 1002
-
 maltose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 1035
-
 allose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 1038
-
 lactose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 1064
-
 3-O-methyl-D-glucose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 1253
-
 3-O-methyl-D-glucose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 1399
-
 D-glucose N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 1659
-
 lactose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 1791
-
 D-glucose N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 1930
-
 maltose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 2509
-
 allose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 3011
-
 3-O-methyl-D-glucose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.5.2 3860
-
 D-glucose wild-type isozyme PQQGDH-B, pH 7.0, 25°C Escherichia coli
1.1.99.35 69
-
 D-galactose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 72
-
 D-galactose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 232
-
 D-galactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 574
-
 D-lactose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 588
-
 D-maltose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 949
-
 D-allose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1002
-
 D-maltose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1035
-
 D-allose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1038
-
 D-lactose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1064
-
 3-O-methyl-D-glucose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1253
-
 3-O-methyl-D-glucose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1399
-
 D-glucose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1659
-
 D-lactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1791
-
 D-glucose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1930
-
 D-maltose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 2509
-
 D-allose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 3011
-
 3-O-methyl-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 3860
-
 D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 7
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone prosthetic group Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.35 22
-
 D-maltose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 23
-
 D-galactose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 25
-
 D-allose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 26
-
 D-galactose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 31
-
 D-lactose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 32
-
 D-allose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 32
-
 D-lactose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 37
-
 D-maltose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 37
-
 3-O-methyl-D-glucose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 44
-
 D-galactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 45
-
 3-O-methyl-D-glucose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 71
-
 D-allose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 74
-
 D-maltose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 88
-
 D-lactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 105
-
 3-O-methyl-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 114
-
 D-glucose mutant N462H, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 143
-
 D-glucose mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 154
-
 D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus