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Literature summary extracted from
- Hydroaromatic metabolism in Rhodococcus rhodochrous: purification and characterization of its NAD-dependent quinate dehydrogenase (1990), Arch. Microbiol., 154, 179-186.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.282 | 0.15 | - |
beta-NAD+ | pH 10, cosubstrate (-)-quinate | Rhodococcus rhodochrous |  |
| 1.1.1.282 | 0.48 | - |
nicotinamide hypoxanthine dinucleotide | pH 10, cosubstrate (-)-quinate | Rhodococcus rhodochrous | |
| 1.1.1.282 | 0.51 | - |
oxidized nicotinamide 1,N6-ethanoadenine dinucleotide | pH 10, cosubstrate (-)-quinate | Rhodococcus rhodochrous | |
| 1.1.1.282 | 0.65 | - |
3-acetylpyridine adenine dinucleotide | pH 10, cosubstrate (-)-quinate | Rhodococcus rhodochrous | |
| 1.1.1.282 | 2.47 | - |
t-3,t-4-dihydroxycyclohexane-c-1-carboxylate | pH 10, (-)-enantiomer, cosubstrate NAD+ | Rhodococcus rhodochrous | |
| 1.1.1.282 | 2.56 | - |
dihydroshikimate | pH 10, (-)-enantiomer, cosubstrate NAD+ | Rhodococcus rhodochrous | |
| 1.1.1.282 | 2.95 | - |
L-quinate | pH 10, (-)-enantiomer, cosubstrate NAD+ | Rhodococcus rhodochrous | |
| 1.1.1.282 | 5.25 | - |
shikimate | pH 10, (-)-enantiomer, cosubstrate NAD+ | Rhodococcus rhodochrous | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.282 | 31500 | - |
1 * 31500, SDS-PAGE | Rhodococcus rhodochrous |
| 1.1.1.282 | 44000 | - |
gel filtration | Rhodococcus rhodochrous |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.282 | additional information | Rhodococcus rhodochrous | initial enzyme of the hydroaromatic pathway | ? | - |
? | |
| 1.1.1.282 | additional information | Rhodococcus rhodochrous N75 | initial enzyme of the hydroaromatic pathway | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.282 | Rhodococcus rhodochrous | - |
- |
- |
| 1.1.1.282 | Rhodococcus rhodochrous N75 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.282 | 188fold | Rhodococcus rhodochrous |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.1.1.282 | L-quinate + NAD(P)+ = 3-dehydroquinate + NAD(P)H + H+ | stereospecificity | Rhodococcus rhodochrous | |
| 1.1.1.282 | shikimate + NAD(P)+ = 3-dehydroshikimate + NAD(P)H + H+ | stereospecificity | Rhodococcus rhodochrous |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.282 | additional information | - |
- |
Rhodococcus rhodochrous |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.282 | dihydroshikimate + NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, 38% of the activity with (-)-quinate | Rhodococcus rhodochrous | (1S,3R,4S)-3,4-dihydroxy-5-oxocyclohexanecarboxylic acid + NADH + H+ | (-)-enantiomer, reverse reaction: 3.3fold higher activity than with (-)-3-dehydroquinate | r | |
| 1.1.1.282 | dihydroshikimate + NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, 38% of the activity with (-)-quinate | Rhodococcus rhodochrous N75 | (1S,3R,4S)-3,4-dihydroxy-5-oxocyclohexanecarboxylic acid + NADH + H+ | (-)-enantiomer, reverse reaction: 3.3fold higher activity than with (-)-3-dehydroquinate | r | |
| 1.1.1.282 | L-quinate + 3-acetylpyridine adenine dinucleotide | 67% of the activity with NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + ? | - |
? | |
| 1.1.1.282 | L-quinate + 3-acetylpyridine adenine dinucleotide | 67% of the activity with NAD+ | Rhodococcus rhodochrous N75 | 3-dehydroquinate + ? | - |
? | |
| 1.1.1.282 | L-quinate + beta-NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, a single enzyme with both quinate and shikimate dehydrogenase activity | Rhodococcus rhodochrous | 3-dehydroquinate + beta-NADH + H+ | (-)-enantiomer, reverse reaction: lower activity than with t-4,c-5-dihydroxy-3-oxocyclohexane-c-1-carboxylate or 4-hydroxy-3-oxocyclohexane-c-1-carboxylate | r | |
| 1.1.1.282 | L-quinate + beta-NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, a single enzyme with both quinate and shikimate dehydrogenase activity | Rhodococcus rhodochrous N75 | 3-dehydroquinate + beta-NADH + H+ | (-)-enantiomer, reverse reaction: lower activity than with t-4,c-5-dihydroxy-3-oxocyclohexane-c-1-carboxylate or 4-hydroxy-3-oxocyclohexane-c-1-carboxylate | r | |
| 1.1.1.282 | L-quinate + NADP+ | 0.3% of the activity with NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + NADPH + H+ | - |
? | |
| 1.1.1.282 | L-quinate + nicotinamide 1,N6-ethenoadenine dinucleotide | 69% of the activity with NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + ? | - |
? | |
| 1.1.1.282 | L-quinate + nicotinamide hypoxanthine dinucleotide | 1.3fold higher activity than with NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + ? | - |
? | |
| 1.1.1.282 | L-quinate + oxidized nicotinamide 1,N6-ethanoadenine dinucleotide | 69% activity compared to NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + reduced nicotinamide 1,N6-ethanoadenine dinucleotide | - |
r | |
| 1.1.1.282 | additional information | initial enzyme of the hydroaromatic pathway | Rhodococcus rhodochrous | ? | - |
? | |
| 1.1.1.282 | additional information | substrate specificity, enzyme is highly stereospecific with regard to hydroaromatic substrates, oxidizing only the axial hydroxyl group at C-3 of (-)-enantiomer of quinate, shikimate, dihydroshikimate and t-3,t-4-dihydroxycyclohexane-c-1-carboxylate, enzyme shows activity with several NAD+ analogues, reverse reaction: not 4-hydroxy-3-oxocyclohex-4-ene-c-1-carboxylate, not: alpha-NAD+, beta-NMN or nicotinic acid dinucleotide | Rhodococcus rhodochrous | ? | - |
? | |
| 1.1.1.282 | additional information | initial enzyme of the hydroaromatic pathway | Rhodococcus rhodochrous N75 | ? | - |
? | |
| 1.1.1.282 | additional information | substrate specificity, enzyme is highly stereospecific with regard to hydroaromatic substrates, oxidizing only the axial hydroxyl group at C-3 of (-)-enantiomer of quinate, shikimate, dihydroshikimate and t-3,t-4-dihydroxycyclohexane-c-1-carboxylate, enzyme shows activity with several NAD+ analogues, reverse reaction: not 4-hydroxy-3-oxocyclohex-4-ene-c-1-carboxylate, not: alpha-NAD+, beta-NMN or nicotinic acid dinucleotide | Rhodococcus rhodochrous N75 | ? | - |
? | |
| 1.1.1.282 | shikimate + NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, a single enzyme with both quinate and shikimate dehydrogenase activity, 72% of the activity with (-)-quinate | Rhodococcus rhodochrous | 3-dehydroshikimate + NADH + H+ | (-)-enantiomer, reverse reaction: 15% of the activity with (-)-3-dehydroquinate | r | |
| 1.1.1.282 | t-3,t-4-dihydroxycyclohexane-c-1-carboxylate + NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, 44% of the activity with (-)-quinate | Rhodococcus rhodochrous | 4-hydroxy-3-oxocyclohexane-c-1-carboxylate + NADH + H+ | (-)-isomer, reverse reaction: 2.2fold higher activity than with (-)-3-dehydroquinate | r | |
| 1.1.1.282 | t-3-hydroxy-4-oxocyclohexane-c-1-carboxylate + NAD+ | 6% of the activity with (-)-quinate | Rhodococcus rhodochrous | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.282 | monomer | 1 * 31500, SDS-PAGE | Rhodococcus rhodochrous |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.282 | 45 | - |
t1/2: 1.85 min | Rhodococcus rhodochrous |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.282 | 7.5 | - |
reduction reaction, assay at | Rhodococcus rhodochrous |
| 1.1.1.282 | 10 | - |
oxidation reaction, quinate or shikimate as substrates, 50 mM glycine-KOH | Rhodococcus rhodochrous |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.282 | 7 | 8.5 | the rate of activity decreases more rapidly, maximal 25% at pH 7.5, at pH values from 8.5 to 7 when shikimate rather than quinate is used as substrate | Rhodococcus rhodochrous |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.282 | 3-acetylpyridine adenine dinucleotide | 67% of the activity with NAD+ | Rhodococcus rhodochrous | |
| 1.1.1.282 | additional information | absolute requirement for a nicotinamide nucleotide cofactor for the oxidation of quinate or shikimate, cofactor specificity, not: alpha-NAD+, beta-NMN or nicotinic acid dinucleotide | Rhodococcus rhodochrous | |
| 1.1.1.282 | NAD+ | - |
Rhodococcus rhodochrous | |
| 1.1.1.282 | NADH | - |
Rhodococcus rhodochrous | |
| 1.1.1.282 | NADP+ | 0.3% of the activity with NAD+ | Rhodococcus rhodochrous | |
| 1.1.1.282 | nicotinamide 1,N6-ethenoadenine dinucleotide | 69% of the activity with NAD+ | Rhodococcus rhodochrous | |
| 1.1.1.282 | nicotinamide hypoxanthine dinucleotide | 1.3fold higher activity than with NAD+ | Rhodococcus rhodochrous | |
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