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Literature summary extracted from

  • Shearer, H.L.; Turpin, D.H.; Dennis, D.T.
    Characterization of NADP-dependent malic enzyme from developing castor oil seed endosperm (2004), Arch. Biochem. Biophys., 429, 134-144.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.40 acetyl carnitine 19% activation at 5 mM Ricinus communis
1.1.1.40 acetyl-CoA 24% activation at 0.005 mM Ricinus communis
1.1.1.40 ATP 18% activation at 5 mM Ricinus communis
1.1.1.40 citrate 22% activation at 5 mM Ricinus communis
1.1.1.40 CoA 29% activation at 0.005 mM Ricinus communis
1.1.1.40 L-glutamate 15% activation at 5 mM Ricinus communis
1.1.1.40 malonyl-CoA 15% activation at 0.005 mM Ricinus communis
1.1.1.40 palmitoyl-CoA 69% activation at 0.005 mM Ricinus communis
1.1.1.40 succinate 65% activation at 5 mM Ricinus communis
1.1.1.40 UDP 10% activation at 5 mM Ricinus communis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 AMP 41% inhibition at 5 mM Ricinus communis
1.1.1.40 cAMP 10% inhibition at 5 mM Ricinus communis
1.1.1.40 CO2 product inhibition, uncompetitive with respect to L-malate and NADP+, 39% inhibition at 25 mM Ricinus communis
1.1.1.40 D-fructose-1,6-bisphosphate 13% inhibition at 5 mM Ricinus communis
1.1.1.40 GDP 13% inhibition at 5 mM Ricinus communis
1.1.1.40 NADPH product inhibition, competitive with respect to L-malate and NADP+ Ricinus communis
1.1.1.40 oxalate 51% inhibition at 1 mM Ricinus communis
1.1.1.40 phosphoenolpyruvate 39% inhibition at 5 mM Ricinus communis
1.1.1.40 pyruvate product inhibition, competitive with respect to L-malate, 16% inhibition at 5 mM Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.0103
-
 NADP+ pH 7.6-7.7, 25°C Ricinus communis
1.1.1.40 0.22
-
 L-malate pH 7.6-7.7, 25°C Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.40 leucoplast
-
 Ricinus communis 9516
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.40 Mg2+ enzyme requires either Mg2+ or Mn2+ for activity, no activity without divalent cation Ricinus communis
1.1.1.40 Mn2+ enzyme requires either Mg2+ or Mn2+ for activity, no activity without divalent cation Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.40 63000
-
 4 * 63000, approximately, SDS-PAGE Ricinus communis
1.1.1.40 254000
-
 gel filtration Ricinus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ Ricinus communis enzyme is involved in fatty acid biosynthesis in oil seed leucoplasts, overview pyruvate + CO2 + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Ricinus communis
-
 cv. Baker 296
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40 5160fold from oil seed Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 endosperm developing, maximal at stage 7 of endosperm development, overview Ricinus communis
-
1.1.1.40 seed
-
 Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.40 18.2
-
 purified enzyme Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ enzyme is involved in fatty acid biosynthesis in oil seed leucoplasts, overview Ricinus communis pyruvate + CO2 + NADPH
-
 r
1.1.1.40 (S)-malate + NADP+ decarboxylation is favoured Ricinus communis pyruvate + CO2 + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.40 tetramer 4 * 63000, approximately, SDS-PAGE Ricinus communis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.40 NADP-dependent malic enzyme
-
 Ricinus communis
1.1.1.40 NADP-ME
-
 Ricinus communis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.40 25
-
 assay at, both reaction directions Ricinus communis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.40 44
-
 stable for at least 10 min Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 7.6 7.7 both reaction directions Ricinus communis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.40 7 8.2 broad range, 80% of maximal activity at pH 7.0 and pH 8.2 Ricinus communis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NADP+ absolutely specific for, no activity with NAD+ Ricinus communis
1.1.1.40 NADPH
-
 Ricinus communis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.40 additional information
-
 additional information inhibition kinetics Ricinus communis
1.1.1.40 0.016
-
 pyruvate pH 7.6-7.7, 25°C Ricinus communis
1.1.1.40 0.046
-
 NADPH versus NADP+, pH 7.6-7.7, 25°C Ricinus communis
1.1.1.40 0.372
-
 CO2 versus NADP+, pH 7.6-7.7, 25°C Ricinus communis
1.1.1.40 0.492
-
 NADPH versus L-malate, pH 7.6-7.7, 25°C Ricinus communis
1.1.1.40 0.83
-
 CO2 versus L-malate, pH 7.6-7.7, 25°C Ricinus communis