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Literature summary extracted from

  • Contreras-Shannon, V.; McAlister-Henn, L.
    Influence of compartmental localization on the function of yeast NADP+-specific isocitrate dehydrogenases (2004), Arch. Biochem. Biophys., 423, 235-246.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.42 additional information isozyme IDP3 is inducible by fatty acids, isozyme IDP2 by non-fermentable carbon sources, expression of isozyme IDP1 is constitutive Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 exchange of promotors and altering of organellar targeting for expression of isozymes IDO2 and IDO3 in mitochondria, and of isozymes IDP1 and IDP3 in the cytosol, functional complementation studies using mutant strains Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.01
-
 NADPH isozyme IDP1 Saccharomyces cerevisiae
1.1.1.42 0.02
-
 NADP+ isozyme IDP2 and IDP3 Saccharomyces cerevisiae
1.1.1.42 0.03
-
 NADP+ isozyme IDP1 Saccharomyces cerevisiae
1.1.1.42 0.03
-
 isocitrate isozyme IDP1 Saccharomyces cerevisiae
1.1.1.42 0.04
-
 NADPH isozyme IDP2 Saccharomyces cerevisiae
1.1.1.42 0.05
-
 isocitrate isozyme IDP3 Saccharomyces cerevisiae
1.1.1.42 0.2
-
 2-oxoglutarate isozyme IDP2 Saccharomyces cerevisiae
1.1.1.42 0.22
-
 isocitrate isozyme IDP2 Saccharomyces cerevisiae
1.1.1.42 1.92
-
 2-oxoglutarate isozyme IDP1 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 cytosol isoyzme IDP2 Saccharomyces cerevisiae 5829
-
1.1.1.42 mitochondrion isozyme IDP1 Saccharomyces cerevisiae 5739
-
1.1.1.42 additional information influence of subcellular localization on isozyme activities, overview Saccharomyces cerevisiae
-
-
1.1.1.42 peroxisome isozyme IDP3 Saccharomyces cerevisiae 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Saccharomyces cerevisiae isozymes are compartment interchangeable for glutamate synthesis, although mitochondrial localization has a positive impact on this function during fermentative growth 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Saccharomyces cerevisiae
-
 3 isozymes: mitochondrial IDP1, cytosolic IDP2, peroxisomal IDP3
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.1.42 isozyme IDP3 shows highest defense against endogeneous oxidative stress Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 additional information
-
 wild-type and diverse mutant strains Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+ isozymes are compartment interchangeable for glutamate synthesis, although mitochondrial localization has a positive impact on this function during fermentative growth Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 isocitrate + NADP+ reaction is reversible for isozymes IDP1 and IDP2 Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 NADP+-specific isocitrate dehydrogenase
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.8
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Saccharomyces cerevisiae
1.1.1.42 NADPH
-
 Saccharomyces cerevisiae