BRENDA - Enzyme Database

Influence of compartmental localization on the function of yeast NADP+-specific isocitrate dehydrogenases

Contreras-Shannon, V.; McAlister-Henn, L.; Arch. Biochem. Biophys. 423, 235-246 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.42
additional information
isozyme IDP3 is inducible by fatty acids, isozyme IDP2 by non-fermentable carbon sources, expression of isozyme IDP1 is constitutive
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
exchange of promotors and altering of organellar targeting for expression of isozymes IDO2 and IDO3 in mitochondria, and of isozymes IDP1 and IDP3 in the cytosol, functional complementation studies using mutant strains
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.01
-
NADPH
isozyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.02
-
NADP+
isozyme IDP2 and IDP3
Saccharomyces cerevisiae
1.1.1.42
0.03
-
isocitrate
isozyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.03
-
NADP+
isozyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.04
-
NADPH
isozyme IDP2
Saccharomyces cerevisiae
1.1.1.42
0.05
-
isocitrate
isozyme IDP3
Saccharomyces cerevisiae
1.1.1.42
0.2
-
2-oxoglutarate
isozyme IDP2
Saccharomyces cerevisiae
1.1.1.42
0.22
-
isocitrate
isozyme IDP2
Saccharomyces cerevisiae
1.1.1.42
1.92
-
2-oxoglutarate
isozyme IDP1
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
isoyzme IDP2
Saccharomyces cerevisiae
5829
-
1.1.1.42
mitochondrion
isozyme IDP1
Saccharomyces cerevisiae
5739
-
1.1.1.42
additional information
influence of subcellular localization on isozyme activities, overview
Saccharomyces cerevisiae
-
-
1.1.1.42
peroxisome
isozyme IDP3
Saccharomyces cerevisiae
5777
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae
isozymes are compartment interchangeable for glutamate synthesis, although mitochondrial localization has a positive impact on this function during fermentative growth
2-oxoglutarate + CO2 + NADPH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Saccharomyces cerevisiae
-
3 isozymes: mitochondrial IDP1, cytosolic IDP2, peroxisomal IDP3
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.1.1.42
isozyme IDP3 shows highest defense against endogeneous oxidative stress
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.42
additional information
-
wild-type and diverse mutant strains
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
isozymes are compartment interchangeable for glutamate synthesis, although mitochondrial localization has a positive impact on this function during fermentative growth
654367
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
reaction is reversible for isozymes IDP1 and IDP2
654367
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.8
-
assay at
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Saccharomyces cerevisiae
1.1.1.42
NADPH
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.42
additional information
isozyme IDP3 is inducible by fatty acids, isozyme IDP2 by non-fermentable carbon sources, expression of isozyme IDP1 is constitutive
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
exchange of promotors and altering of organellar targeting for expression of isozymes IDO2 and IDO3 in mitochondria, and of isozymes IDP1 and IDP3 in the cytosol, functional complementation studies using mutant strains
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Saccharomyces cerevisiae
1.1.1.42
NADPH
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.01
-
NADPH
isozyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.02
-
NADP+
isozyme IDP2 and IDP3
Saccharomyces cerevisiae
1.1.1.42
0.03
-
isocitrate
isozyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.03
-
NADP+
isozyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.04
-
NADPH
isozyme IDP2
Saccharomyces cerevisiae
1.1.1.42
0.05
-
isocitrate
isozyme IDP3
Saccharomyces cerevisiae
1.1.1.42
0.2
-
2-oxoglutarate
isozyme IDP2
Saccharomyces cerevisiae
1.1.1.42
0.22
-
isocitrate
isozyme IDP2
Saccharomyces cerevisiae
1.1.1.42
1.92
-
2-oxoglutarate
isozyme IDP1
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
isoyzme IDP2
Saccharomyces cerevisiae
5829
-
1.1.1.42
mitochondrion
isozyme IDP1
Saccharomyces cerevisiae
5739
-
1.1.1.42
additional information
influence of subcellular localization on isozyme activities, overview
Saccharomyces cerevisiae
-
-
1.1.1.42
peroxisome
isozyme IDP3
Saccharomyces cerevisiae
5777
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae
isozymes are compartment interchangeable for glutamate synthesis, although mitochondrial localization has a positive impact on this function during fermentative growth
2-oxoglutarate + CO2 + NADPH
-
-
?
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.1.1.42
isozyme IDP3 shows highest defense against endogeneous oxidative stress
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.42
additional information
-
wild-type and diverse mutant strains
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
isozymes are compartment interchangeable for glutamate synthesis, although mitochondrial localization has a positive impact on this function during fermentative growth
654367
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
reaction is reversible for isozymes IDP1 and IDP2
654367
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.8
-
assay at
Saccharomyces cerevisiae