EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.1 | expression in Sf9 insect cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.1 | crystals of wild-type and F256W mutant alpha-amylase, crystals diffract to 2.1 a resolution | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 0.06 | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 37°C, pH 8.0, F256W mutant | Homo sapiens | |
3.2.1.1 | 0.27 | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 37°C, pH 8.0, wild-type | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 55000 | - |
x * 55000, non-glycosylated alpha-amylase | Homo sapiens |
3.2.1.1 | 62000 | - |
x * 62000, glycosylated alpha-amylase | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | starch + H2O | Homo sapiens | - |
fragments of starch | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.1 | glycoprotein | human salivary alpha-amylase occurs in both glycosylated and non-glycosylated forms | Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | recombinant alpha-amylase | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.1 | saliva | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | additional information | - |
F256W mutant, 900 units, 1 unit is definded as the amount of alpha-amylase able to decrease 1% of absorbance at 620 nm in 1 min | Homo sapiens |
3.2.1.1 | additional information | - |
wild-type, 66400 units, 1 unit is definded as the amount of alpha-amylase able to decrease 1% of absorbance at 620 nm in 1 min | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + H2O | - |
Homo sapiens | ? | - |
? | |
3.2.1.1 | starch + H2O | - |
Homo sapiens | fragments of starch | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.1 | ? | x * 55000, non-glycosylated alpha-amylase | Homo sapiens |
3.2.1.1 | ? | x * 62000, glycosylated alpha-amylase | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 8.5 | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 37°C, pH 8.0, F256W mutant | Homo sapiens | |
3.2.1.1 | 175 | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 37°C, pH 8.0, wild-type | Homo sapiens |