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Literature summary extracted from

  • Ramasubbu, N.; Sundar, K.; Ragunath, C.; Rafi, M.M.
    Structural studies of a Phe256Trp mutant of human salivary alpha-amylase: implications for the role of a conserved water molecule in enzyme activity (2004), Arch. Biochem. Biophys., 421, 115-124.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Sf9 insect cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.1 crystals of wild-type and F256W mutant alpha-amylase, crystals diffract to 2.1 a resolution Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 0.06
-
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 8.0, F256W mutant Homo sapiens
3.2.1.1 0.27
-
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 8.0, wild-type Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 55000
-
x * 55000, non-glycosylated alpha-amylase Homo sapiens
3.2.1.1 62000
-
x * 62000, glycosylated alpha-amylase Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Homo sapiens
-
fragments of starch
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.1 glycoprotein human salivary alpha-amylase occurs in both glycosylated and non-glycosylated forms Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant alpha-amylase Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 saliva
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 additional information
-
F256W mutant, 900 units, 1 unit is definded as the amount of alpha-amylase able to decrease 1% of absorbance at 620 nm in 1 min Homo sapiens
3.2.1.1 additional information
-
wild-type, 66400 units, 1 unit is definded as the amount of alpha-amylase able to decrease 1% of absorbance at 620 nm in 1 min Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + H2O
-
Homo sapiens ?
-
?
3.2.1.1 starch + H2O
-
Homo sapiens fragments of starch
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 55000, non-glycosylated alpha-amylase Homo sapiens
3.2.1.1 ? x * 62000, glycosylated alpha-amylase Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 8.5
-
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 8.0, F256W mutant Homo sapiens
3.2.1.1 175
-
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 8.0, wild-type Homo sapiens