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Literature summary extracted from

  • Cho, H.; Oliveira, M.A.; Tai, H.H.
    Critical residues for the coenzyme specificity of NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (2003), Arch. Biochem. Biophys., 419, 139-146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.141 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.141 A13G/A14S/Q15K/D36S/W37R no activity Homo sapiens
1.1.1.141 A14S/Q15K/D36S/W37R no activity Homo sapiens
1.1.1.141 Q15K approx. 200% of wild-type activity Homo sapiens
1.1.1.141 Q15K/D36A/W37R no activity with NAD+ as cofactor, low activity with NADP+ as cofactor Homo sapiens
1.1.1.141 Q15K/D36S/W37R no activity with NAD+ as cofactor, low activity with NADP+ as cofactor Homo sapiens
1.1.1.141 Q15K/W37K approx. 350% of wild-type activity Homo sapiens
1.1.1.141 Q15K/W37R approx. 350% of wild-type activity, strong activity with NADP+ as cofactor Homo sapiens
1.1.1.141 Q15R approx. 100% of wild-type activity Homo sapiens
1.1.1.141 Q15R/W37K approx. 200% of wild-type activity Homo sapiens
1.1.1.141 Q15R/W37R approx. 200% of wild-type activity, strong activity with NADP+ as cofactor Homo sapiens
1.1.1.141 W37K approx. 100% of wild-type activity Homo sapiens
1.1.1.141 W37R approx. 100% of wild-type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.141 0.0063
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5 Homo sapiens
1.1.1.141 0.013
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, Q15K/W37K double mutant Homo sapiens
1.1.1.141 0.026
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, Q15K mutant Homo sapiens
1.1.1.141 0.032
-
 NAD+ 37°C, pH 7.5 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ Homo sapiens
-
 (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.141 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.141 recombinant 15-PGDH Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.141 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+
-
 Homo sapiens (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.141 15-PGDH
-
 Homo sapiens