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Literature summary extracted from

  • Pollock, V.V.; Conover, R.C.; Johnson, M.K.; Barber, M.J.
    Bacterial expression of the molybdenum domain of assimilatory nitrate reductase: production of both the functional molybdenum-containing domain and the nonfunctional tungsten analog (2002), Arch. Biochem. Biophys., 403, 237-248.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.1 expression of amino-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein Spinacia oleracea

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.1 additional information expression of 541 residue amonio-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.008
-
 nitrate His-tagged molybdenum domain, 25°C, pH 7.6 Spinacia oleracea
1.7.1.1 0.012
-
 nitrate GST-tagged molybdenum domain, 25°C, pH 7.6 Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.1 Molybdenum Mo/protein ratio 0.44 for His-tagged protein, 0.93 for GST-tagged protein Spinacia oleracea
1.7.1.1 additional information tungsten cannot replace for molybdenum in the GST-tagged protein Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.1 55000
-
 x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain Spinacia oleracea
1.7.1.1 85000
-
 x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Spinacia oleracea
-
 expression of amino-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 0.0015
-
 His-tagged molybdenum domain, 25°C, pH 7.6 Spinacia oleracea
1.7.1.1 0.0108
-
 GST-tagged molybdenum domain, 25°C, pH 7.6 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 reduced methyl viologen + nitrate
-
 Spinacia oleracea methyl viologen + nitrite
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.1 ? x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain Spinacia oleracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.1 1.8
-
 nitrate GST-tagged molybdenum domain, 25°C, pH 7.6 Spinacia oleracea
1.7.1.1 2.5
-
 nitrate His-tagged molybdenum domain, 25°C, pH 7.6 Spinacia oleracea