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Literature summary extracted from

  • Zheng, W.; Wise, M.L.; Wyrick, A.; Metz, J.G.; Yuan, L.; Gerwick, W.H.
    Polyenoic fatty acid isomerase from the marine alga Ptilota filicina: protein characterization and functional expression of the cloned cDNA (2002), Arch. Biochem. Biophys., 401, 11-20.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.13 cDNA, expression in Arabidopsis thaliana Ptilota filicina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.13 53600
-
 2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide Ptilota filicina
5.3.3.13 55900
-
 2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide Ptilota filicina
5.3.3.13 61000
-
 2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide Ptilota filicina
5.3.3.13 125000
-
 sedimentation equilibrium utracentrifugation Ptilota filicina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.13 additional information Ptilota filicina PFI may be part of a stress tolerance mechanism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.13 Ptilota filicina Q8W257
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.3.3.13 glycoprotein native PFI is N-glycosylated Ptilota filicina
5.3.3.13 proteolytic modification translation of a preprotein containing a signal peptide of 21 amino acids that is removed during maturation Ptilota filicina

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.13
-
 Ptilota filicina

Storage Stability

EC Number Storage Stability Organism
5.3.3.13 -20°C, purified PFI, months, stable Ptilota filicina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.13 (5Z,8Z,11Z,14Z)-eicosatetraenoate arachidonic acid, conversion to the conjugated triene Ptilota filicina ?
-
 ?
5.3.3.13 (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate the preferred substrates are highly unsaturated free fatty acids such as eicosapentaenoic acid Ptilota filicina (5Z,7E,9E,14Z,17Z)-eicosapentaenoate
-
 ?
5.3.3.13 additional information PFI may be part of a stress tolerance mechanism Ptilota filicina ?
-
 ?
5.3.3.13 polyenoic fatty acid PFI catalyzes the isomerization of a wide range of substrates containing three or more methylene interrupted olefins into a Z,E,E conjugated triene functionality Ptilota filicina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.3.13 homodimer 2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide Ptilota filicina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.3.13 22
-
 assay at Ptilota filicina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.13 7.2
-
 assay at Ptilota filicina

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.3.13 flavin bound to PFI, cDNA contains a flavin-binding motif near the mature N-terminus Ptilota filicina

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.3.13 Ptilota filicina sequence calculation including the signal peptide
-
 4.9
5.3.3.13 Ptilota filicina sequence calculation without the signal peptide
-
 4.9