EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.74 | 2-ethyl-5-phenylisoxazolium-3'-sulfonate | inhibition is potentiated by MgCl2 | Homo sapiens | |
3.1.3.74 | 5,5'-dithiobis(2-nitrobenzoate) | inhibition is potentiated by MgCl2 | Homo sapiens | |
3.1.3.74 | Ca2+ | competitive inhibition versus Mg2+, noncompetitive versus substrate | Homo sapiens | |
3.1.3.74 | Cd2+ | - |
Homo sapiens | |
3.1.3.74 | Cu2+ | - |
Homo sapiens | |
3.1.3.74 | diethyldicarbonate | inhibition is not potentiated by MgCl2 | Homo sapiens | |
3.1.3.74 | Fe2+ | - |
Homo sapiens | |
3.1.3.74 | iodoacetamide | weak | Homo sapiens | |
3.1.3.74 | iodoacetate | inhibition is potentiated by MgCl2 | Homo sapiens | |
3.1.3.74 | Mn2+ | inhibits above 0.05 mM, activates below 0.05 mM | Homo sapiens | |
3.1.3.74 | molybdate | competitive inhibition versus substrate, noncompetitive versus Mg2+ | Homo sapiens | |
3.1.3.74 | N-ethylmaleimide | inactivated by low concentrations, low concentrations of a substrate, pyridoxine phosphate, or phosphate protect from inactivation, inhibition is not potentiated by MgCl2 | Homo sapiens | |
3.1.3.74 | Pb2+ | - |
Homo sapiens | |
3.1.3.74 | Phenylglyoxal | inhibition is not potentiated by MgCl2 | Homo sapiens | |
3.1.3.74 | phosphate | competitive inhibition versus substrate, noncompetitive versus Mg2+ | Homo sapiens | |
3.1.3.74 | Zn2+ | inhibits at higher concentrations, activates somewhat at low concentrations | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.74 | additional information | - |
additional information | kinetic mechanism, kinetic properties | Homo sapiens | |
3.1.3.74 | 0.00154 | - |
pyridoxal 5'-phosphate | pH 7.4, 37°C, 0.188 mM Mg2+ | Homo sapiens | |
3.1.3.74 | 0.00166 | - |
pyridoxal 5'-phosphate | pH 7.4, 37°C, 0.06 mM Co2+ | Homo sapiens | |
3.1.3.74 | 0.0043 | - |
pyridoxine 5'-phosphate | pH 7.4, 37°C, 0.39 mM Mg2+ | Homo sapiens | |
3.1.3.74 | 0.00812 | - |
pyridoxine 5'-phosphate | pH 7.4, 37°C, 0.13 mM Co2+ | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.74 | Co2+ | similar activation as by Mg2+ and Ni2+, kinetics | Homo sapiens | |
3.1.3.74 | Mg2+ | similar activation as by Co2+ and Ni2+, kinetics, probably the physiological activator | Homo sapiens | |
3.1.3.74 | Mn2+ | less activatory than Mg2+, Co2+ or Ni2+, inhibits above 0.05 mM, kinetics | Homo sapiens | |
3.1.3.74 | additional information | essential requirement for divalent cations | Homo sapiens | |
3.1.3.74 | Ni2+ | similar activation as by Mg2+ and Co2+, kinetics | Homo sapiens | |
3.1.3.74 | Zn2+ | activates somewhat at low concentrations, inhibits at higher concentrations | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.74 | 32000 | - |
2 * 32000 | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.74 | additional information | Homo sapiens | probably plays an important role in the regulation of vitamin B6 metabolism | ? | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.3.74 | Ethanol | 10%, no effect on enzyme activity | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.74 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.74 | - |
Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.3.74 | pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate | kinetic mechanism | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.74 | erythrocyte | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.74 | 5.3 | - |
pH 7.4, 37°C | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.74 | additional information | probably plays an important role in the regulation of vitamin B6 metabolism | Homo sapiens | ? | - |
? | |
3.1.3.74 | pyridoxal 5'-phosphate + H2O | kinetic mechanism, random binding of pyridoxal phosphate and Mg2+, formation of a dead-end complex of phosphate with the enzyme-Mg complex | Homo sapiens | pyridoxal + phosphate | - |
? | |
3.1.3.74 | pyridoxine 5'-phosphate + H2O | - |
Homo sapiens | pyridoxine + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.74 | dimer | 2 * 32000 | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.74 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.74 | 7.4 | - |
assay at | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.74 | additional information | - |
additional information | - |
Homo sapiens | |
3.1.3.74 | 0.00348 | - |
molybdate | - |
Homo sapiens | |
3.1.3.74 | 0.00638 | - |
Ca2+ | - |
Homo sapiens | |
3.1.3.74 | 0.22 | - |
phosphate | - |
Homo sapiens |