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Literature summary extracted from

  • Jourdan, P.S.; Mansell, R.L.
    Isolation and partial characterization of three glucosyl transferases involved in the biosynthesis of flavonol triglucosides in Pisum sativum L (1982), Arch. Biochem. Biophys., 213, 434-443.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.239 additional information not inhibited by divalent cations, 0.1-10 mM, EDTA, EGTA, no substrate inhibition Pisum sativum
2.4.1.239 UDP product inhibition, 1.75 mM, 50% inhibition Pisum sativum
2.4.1.240 additional information not inhibited by divalent cations, 0.1-10 mM, EDTA, EGTA, no substrate inhibition Pisum sativum
2.4.1.240 UDP product inhibition, 0.25 mM, 50% inhibition Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.239 0.004
-
 kaempferol 3-O-beta-D-glucoside pH 7.3, 30°C Pisum sativum
2.4.1.239 0.64
-
 UDP-glucose pH 7.3, 30°C Pisum sativum
2.4.1.240 0.008
-
 kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside pH 7.3, 30°C Pisum sativum
2.4.1.240 0.8
-
 UDP-glucose pH 7.3, 30°C Pisum sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.239 additional information not activated by divalent cations, 0.1-10 mM Pisum sativum
2.4.1.240 additional information not activated by divalent cations, 0.1-10 mM Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.239 additional information Pisum sativum one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides ?
-
 ?
2.4.1.240 additional information Pisum sativum one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.239 Pisum sativum
-
 var. Alaska
-
2.4.1.240 Pisum sativum
-
 var. Alaska
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.239 331fold, separation of three distinct flavonol:glucosyltransferases Pisum sativum
2.4.1.240 30.7fold, separation of three distinct flavonol:glucosyltransferases Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.239 seedling light-grown, one week old Pisum sativum
-
2.4.1.240 seedling light-grown, one week old Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.239 additional information
-
-
 Pisum sativum
2.4.1.240 additional information
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.4.1.239 -15°C or room temperature, partially purified enzyme, 36 h, 80% loss of activity Pisum sativum
2.4.1.239 4°C, 2 weeks, stable Pisum sativum
2.4.1.240 -15°C or room temperature, partially purified enzyme, 36 h, 80% loss of activity Pisum sativum
2.4.1.240 4°C, 2 weeks, stable Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.239 additional information one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides Pisum sativum ?
-
 ?
2.4.1.239 additional information no glucosylation of pelargonidin, naringenin, naringin and p-coumaric acid by the three flavonol:glucosyltransferases, not: ADP-glucose, GDP-glucose, kaempferol, kaempferol diglucoside, quercetin-3-rutinoside, quercetin-3-rhamnoside Pisum sativum ?
-
 ?
2.4.1.239 TDP-glucose + a flavonol 3-O-beta-D-glucoside 3fold lower rate than with UDP-glucose as glucosyl donor Pisum sativum TDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.239 UDP-glucose + a flavonol 3-O-beta-D-glucoside highly specific enzyme, specific for flavonol-3-O-monoglucosides, 3fold higher rate than with TDP-glucose as glucosyl donor Pisum sativum UDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.239 UDP-glucose + kaempferol 3-O-beta-D-glucoside best substrate Pisum sativum UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.239 UDP-glucose + myricetin 3-O-beta-D-glucoside 50% of the activity with kaempferol 3-O-beta-D-glucoside Pisum sativum UDP + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.239 UDP-glucose + pelargonidin 3-O-beta-D-glucoside 2% of the activity with kaempferol 3-O-beta-D-glucoside Pisum sativum UDP + pelargonidin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.239 UDP-glucose + quercetin 3-O-beta-D-glucoside 62% of the activity with kaempferol 3-O-beta-D-glucoside Pisum sativum UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.240 additional information one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides Pisum sativum ?
-
 ?
2.4.1.240 additional information no glucosylation of pelargonidin, naringenin, naringin and p-coumaric acid by the three flavonol:glucosyltransferases, not: ADP-glucose, GDP-glucose, kaempferol, kaempferol monoglucoside, quercetin-3-rutinoside Pisum sativum ?
-
 ?
2.4.1.240 TDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside 3fold lower rate than with UDP-glucose as glucosyl donor Pisum sativum TDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.240 UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside highly specific enzyme, specific for flavonol-3-O-diglucosides, 3fold higher rate than with TDP-glucose as glucosyl donor Pisum sativum UDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.240 UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside best substrate Pisum sativum UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.240 UDP-glucose + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside 32% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside Pisum sativum UDP + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
2.4.1.240 UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside 14% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside Pisum sativum UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.239 UDP-glucose:glucosyltransferase
-
 Pisum sativum
2.4.1.240 UDP-glucose:glucosyltransferase
-
 Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.239 30
-
 assay at Pisum sativum
2.4.1.240 30
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.239 8 9 0.2 M glycine-NaOH buffer, lower activity in 0.2 M Tris-HCl buffer Pisum sativum
2.4.1.240 7.6
-
 in 0.2 M Tris-HCl buffer, two pH optima, equally active in 0.2 M Tris-HCl and 0.2 M glycine-NaOH buffer Pisum sativum
2.4.1.240 8.3
-
 in 0.2 M glycine-NaOH buffer, two pH optima, equally active in 0.2 M Tris-HCl and 0.2 M glycine-NaOH buffer Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.239 additional information no cofactor requirement Pisum sativum
2.4.1.240 additional information no cofactor requirement Pisum sativum