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Literature summary extracted from

  • Uchida, H.; Fukuda, T.; Satoh, Y.; Okamura, Y.; Toriyama, A.; Yamashita, A.; Aisaka, K.; Sakurai, T.; Nagaosa, Y.; Uwajima, T.
    Characterization and potential application of purified aldehyde oxidase from Pseudomonas stutzeri IFO12695 (2005), Appl. Microbiol. Biotechnol., 68, 53-56.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.1 0.22
-
 n-hexylaldehyde 37°C, pH 7.0 Pseudomonas stutzeri
1.2.3.1 29
-
 formaldehyde 37°C, pH 7.0 Pseudomonas stutzeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.1 Iron 3.3 mol Fe per mol protein Pseudomonas stutzeri
1.2.3.1 Molybdenum 0.34 mol Mo per mol protein Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.1 18000
-
 x * 83000 + x * 38000 + x * 18000, SDS-PAGE Pseudomonas stutzeri
1.2.3.1 38000
-
 x * 83000 + x * 38000 + x * 18000, SDS-PAGE Pseudomonas stutzeri
1.2.3.1 83000
-
 x * 83000 + x * 38000 + x * 18000, SDS-PAGE Pseudomonas stutzeri
1.2.3.1 160000
-
 native PAGE Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.1 Pseudomonas stutzeri
-
 12695
-
1.2.3.1 Pseudomonas stutzeri 12695
-
 12695
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.1
-
 Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.1 2-methylbenzaldehyde + H2O + O2 7% of activity with n-hexylaldehyde Pseudomonas stutzeri 2-methylbenzoate + H2O2
-
 ?
1.2.3.1 2-methylbenzaldehyde + H2O + O2 7% of activity with n-hexylaldehyde Pseudomonas stutzeri 12695 2-methylbenzoate + H2O2
-
 ?
1.2.3.1 3-methylbenzaldehyde + H2O + O2 42% of activity with n-hexylaldehyde Pseudomonas stutzeri 3-methylbenzoate + H2O2
-
 ?
1.2.3.1 3-methylbenzaldehyde + H2O + O2 42% of activity with n-hexylaldehyde Pseudomonas stutzeri 12695 3-methylbenzoate + H2O2
-
 ?
1.2.3.1 4-methylbenzaldehyde + H2O + O2 40% of activity with n-hexylaldehyde Pseudomonas stutzeri 4-methylbenzoate + H2O2
-
 ?
1.2.3.1 4-methylbenzaldehyde + H2O + O2 40% of activity with n-hexylaldehyde Pseudomonas stutzeri 12695 4-methylbenzoate + H2O2
-
 ?
1.2.3.1 acetaldehyde + H2O + O2 51% of activity with n-hexylaldehyde Pseudomonas stutzeri acetate + H2O2
-
 ?
1.2.3.1 acetaldehyde + H2O + O2 51% of activity with n-hexylaldehyde Pseudomonas stutzeri 12695 acetate + H2O2
-
 ?
1.2.3.1 benzaldehyde + H2O + O2 41% of activity with n-hexylaldehyde Pseudomonas stutzeri benzoate + H2O2
-
 ?
1.2.3.1 citral + H2O + O2 13% of activity with n-hexylaldehyde Pseudomonas stutzeri (2E)-3,7-dimethylocta-2,6-dienoic acid + H2O2
-
 ?
1.2.3.1 crotonaldehyde + H2O + O2 32% of activity with n-hexylaldehyde Pseudomonas stutzeri crotonate + H2O2
-
 ?
1.2.3.1 DL-glyceraldehyde + H2O + O2 5% of activity with n-hexylaldehyde Pseudomonas stutzeri DL-glycerate + H2O2
-
 ?
1.2.3.1 formaldehyde + H2O + O2 2% of activity with n-hexylaldehyde, aldehyde oxidase might be effective for the removal of formaldehyde contained in wastewater Pseudomonas stutzeri formate + H2O2
-
 ?
1.2.3.1 glutaraldehyde + H2O + O2 30% of activity with n-hexylaldehyde Pseudomonas stutzeri glutarate + H2O2
-
 ?
1.2.3.1 n-heptylaldehyde + H2O + O2 78% of activity with n-hexylaldehyde Pseudomonas stutzeri heptanoate + H2O2
-
 ?
1.2.3.1 n-hexylaldehyde + H2O + O2
-
 Pseudomonas stutzeri hexanoate + H2O2
-
 ?
1.2.3.1 phthalaldehyde + H2O + O2 13% of activity with n-hexylaldehyde Pseudomonas stutzeri phthalic acid + H2O2
-
 ?
1.2.3.1 salicylaldehyde + H2O + O2 8% of activity with n-hexylaldehyde Pseudomonas stutzeri salicylic acid + H2O2
-
 ?
1.2.3.1 salicylaldehyde + H2O + O2 8% of activity with n-hexylaldehyde Pseudomonas stutzeri 12695 salicylic acid + H2O2
-
 ?
1.2.3.1 terephthalaldehyde + H2O + O2 45% of activity with n-hexylaldehyde Pseudomonas stutzeri terephthalic acid + H2O2
-
 ?
1.2.3.1 valeraldehyde + H2O + O2 67% of activity with n-hexylaldehyde Pseudomonas stutzeri valerate + H2O2
-
 ?
1.2.3.1 vanillin + H2O + O2 17% of activity with n-hexylaldehyde Pseudomonas stutzeri vanillic acid + H2O2
-
 ?
1.2.3.1 veratraldehyde + H2O + O2 18% of activity with n-hexylaldehyde Pseudomonas stutzeri veratric acid + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.3.1 ? x * 83000 + x * 38000 + x * 18000, SDS-PAGE Pseudomonas stutzeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.3.1 37
-
-
 Pseudomonas stutzeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.1 60
-
 4% loss of activity after 30 min Pseudomonas stutzeri
1.2.3.1 70
-
 8% loss of activity after 30 min Pseudomonas stutzeri
1.2.3.1 80
-
 50% loss of activity after 30 min Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.1 7
-
-
 Pseudomonas stutzeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.3.1 6 10 more than 90% activity Pseudomonas stutzeri