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Literature summary extracted from

  • Bastian, S.; Rekowski, M.J.; Witte, K.; Heckmann-Pohl, D.M.; Giffhorn, F.
    Engineering of pyranose 2-oxidase from Peniophora gigantea towards improved thermostability and catalytic efficiency (2005), Appl. Microbiol. Biotechnol., 67, 654-663.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.10 synthesis application of the engineered enzyme K312E in bioconversion of L-sorbose to 5-keto-D-fructose Phlebiopsis gigantea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 expression in Escherichia coli BL21(DE3), yielding an intracellular and enzymatically active P2OxB with a volumetric yield of 500 units/ml Phlebiopsis gigantea

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.10 E540K mutant with increased thermo- and pH-stability compared with wild-type, concomitantly with increased catalytic efficiencies (turnover number/KM-value) for D-xylose and L-sorbose Phlebiopsis gigantea
1.1.3.10 K312E mutant enzyme with significant improvements with respect to the ratio of turnover number to Km-value for D-glucose (5.3fold), methyl-beta-D-glucoside (2.0fold), D-galactose (4.8fold), D-xylose (59.9fold), and L-sorbose (69.9fold) compared with wild-type P2Ox Phlebiopsis gigantea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.4
-
D-glucose pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 0.6
-
D-glucose pH 7.3, 22°C, mutant enzyme E540K Phlebiopsis gigantea
1.1.3.10 0.6
-
D-glucose pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 0.8
-
D-glucose pH 7.3, 22°C, recombinant wild-type enzyme Phlebiopsis gigantea
1.1.3.10 1.1
-
D-glucose pH 7.3, 22°C, wild-type enzyme Phlebiopsis gigantea
1.1.3.10 2.4
-
D-xylose pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 2.9
-
L-sorbose pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 5
-
L-sorbose pH 7.3, 22°C, mutant enzyme E540K Phlebiopsis gigantea
1.1.3.10 5.2
-
D-galactose pH 7.3, 22°C, mutant enzyme E540K Phlebiopsis gigantea
1.1.3.10 5.4
-
L-sorbose pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 6.1
-
D-galactose pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 6.3
-
D-galactose pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 6.6
-
D-xylose pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 7.9
-
D-xylose pH 7.3, 22°C, mutant enzyme E540K Phlebiopsis gigantea
1.1.3.10 8.2
-
D-galactose pH 7.3, 22°C, wild-type enzyme Phlebiopsis gigantea
1.1.3.10 11.3
-
2-deoxy-D-glucose pH 7.3, 22°C, wild-type enzyme Phlebiopsis gigantea
1.1.3.10 22.1
-
D-allose pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 22.3
-
D-allose pH 7.3, 22°C, mutant enzyme E540K Phlebiopsis gigantea
1.1.3.10 25
-
D-xylose pH 7.3, 22°C, recombinant wild-type enzyme Phlebiopsis gigantea
1.1.3.10 29.4
-
D-xylose pH 7.3, 22°C, wild-type enzyme Phlebiopsis gigantea
1.1.3.10 47
-
L-sorbose pH 7.3, 22°C, recombinant wild-type enzyme Phlebiopsis gigantea
1.1.3.10 50
-
methyl-beta-D-glucoside pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 50
-
L-sorbose pH 7.3, 22°C, wild-type enzyme Phlebiopsis gigantea
1.1.3.10 55.7
-
D-allose pH 7.3, 22°C, wild-type enzyme Phlebiopsis gigantea
1.1.3.10 68
-
D-allose pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 79
-
2-deoxy-D-glucose pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 99.3
-
2-deoxy-D-glucose pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 256
-
methyl-beta-D-glucoside pH 7.3, 22°C, mutant enzyme E540K Phlebiopsis gigantea
1.1.3.10 261
-
methyl-beta-D-glucoside pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 289
-
methyl-beta-D-glucoside pH 7.3, 22°C, wild-type enzyme Phlebiopsis gigantea

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Phlebiopsis gigantea Q6UG02
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 recombinant wild-type and mutant enzymes Phlebiopsis gigantea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 2.74
-
recombinant wild-type enzyme Phlebiopsis gigantea
1.1.3.10 13.4
-
mutant enzyme E540K with a C-terminal His6-tag Phlebiopsis gigantea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 2-deoxy-D-glucose + O2
-
Phlebiopsis gigantea 2-deoxy-3-dehydro-D-glucose + H2O2
-
r
1.1.3.10 D-allose + O2
-
Phlebiopsis gigantea ?
-
?
1.1.3.10 D-galactose + O2
-
Phlebiopsis gigantea ?
-
?
1.1.3.10 D-glucose + O2
-
Phlebiopsis gigantea 2-dehydro-D-glucose + H2O2
-
?
1.1.3.10 D-xylose + O2
-
Phlebiopsis gigantea ?
-
?
1.1.3.10 L-sorbose + O2
-
Phlebiopsis gigantea ?
-
?
1.1.3.10 methyl-beta-D-glucoside + O2
-
Phlebiopsis gigantea ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 P2Ox
-
Phlebiopsis gigantea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.10 45
-
wild-type enzyme Phlebiopsis gigantea
1.1.3.10 50
-
mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.10 30 58 30°C: about 60% of maximal, 58°C: about 70% of maximal activity, mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea
1.1.3.10 30 53 30°C: about 60% of maximal activity, 58°C: about 75% of maximal activity Phlebiopsis gigantea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 40
-
pH 7.0, 30 min, wild-type enzyme, mutant enzyme K312E with a C-terminal His6-tag and mutant enzyme E540K are stable Phlebiopsis gigantea
1.1.3.10 50
-
pH 7.0, 30 min, wild-type enzyme loses about 40% of its activity, mutant enzyme K312E with a C-terminal His6-tag and mutant enzyme E540K are stable Phlebiopsis gigantea
1.1.3.10 55
-
pH 7.0, 30 min, wild-type enzyme loses 95% of its activity, mutant enzyme K312E with a C-treminal His6-tag and mutant enzyme E540K lose about 10% of its activity Phlebiopsis gigantea
1.1.3.10 60
-
pH 7.0, 30 min, wild-type enzyme completely loses its activity, mutant enzyme K312E with a C-terminal His6-tag loses about 40% of its activity and mutant enzyme E540K loses about 10% of its activity Phlebiopsis gigantea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 5 6 mutant enzyme E540K Phlebiopsis gigantea
1.1.3.10 6
-
mutant enzyme K312E with a C-terminal His6-tag Phlebiopsis gigantea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.10 1 2 pH 7.0, 30 min, wild-type enzyme loses about 75% of its activity, mutant enzyme K312E with a C-terminal His6-tag loses about 70% of its activity and mutant enzyme E540K loses about 10% of its activity Phlebiopsis gigantea
1.1.3.10 6
-
30 min, 30 min, wild-type enzyme and mutant enzyme E540K are stable, mutant enzyme K312E with a C-terminal His6-tag loses about 10% of its activity Phlebiopsis gigantea
1.1.3.10 7 9 30 min, wild-type enzyme, mutant enzyme K312E with a C-terminal His6-tag and mutant enzyme E540K are stable Phlebiopsis gigantea
1.1.3.10 10
-
pH 7.0, 30 min, wild-type enzyme loses about 20% of its activity, mutant enzyme K312E with a C-terminal His6-tag and mutant enzyme E540K are stable Phlebiopsis gigantea
1.1.3.10 11
-
pH 7.0, 30 min, wild-type enzyme loses about 40% of its activity, mutant enzyme K312E with a C-terminal His6-tag loses about 10% of its activity, mutant enzyme E540K is stable Phlebiopsis gigantea