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Literature summary extracted from

  • Hatada, Y.; Hidaka, Y.; Nogi, Y.; Uchimura, K.; Katayama, K.; Li, Z.; Akita, M.; Ohta, Y.; Goda, S.; Ito, H.; Matsui, H.; Ito, S.; Horikoshi, K.
    Hyper-production of an isomalto-dextranase of an Arthrobacter sp. by a proteases-deficient Bacillus subtilis: sequencing, properties, and crystallization of the recombinant enzyme (2004), Appl. Microbiol. Biotechnol., 65, 583-592.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.94 expressed in Bacillus subtilis Arthrobacter dextranolyticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.94 sitting drop vapor diffusion method Arthrobacter dextranolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.94 4-chloromercuribenzoate 53% inactivation at 1 mM, 93% inactivation at 5 mM Arthrobacter dextranolyticus
3.2.1.94 Fe3+ 48% inactivation at 25 mM Arthrobacter dextranolyticus
3.2.1.94 Hg2+ complete inactivation at 25 mM Arthrobacter dextranolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.94 5.4
-
 Dextran 60°C, pH 3.5 Arthrobacter dextranolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.94 Co2+ moderate activation Arthrobacter dextranolyticus
3.2.1.94 Li+ moderate activation Arthrobacter dextranolyticus
3.2.1.94 Mn2+ moderate activation Arthrobacter dextranolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.94 65500
-
 SDS-PAGE Arthrobacter dextranolyticus
3.2.1.94 65990
-
 calculated from amino acid sequence Arthrobacter dextranolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.94 Arthrobacter dextranolyticus Q6BE01
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.94 95% pure Arthrobacter dextranolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.94 43
-
-
 Arthrobacter dextranolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.94 dextran + H2O
-
 Arthrobacter dextranolyticus alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.94 IMD
-
 Arthrobacter dextranolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.94 60
-
-
 Arthrobacter dextranolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.94 50
-
 stable at pH 5 Arthrobacter dextranolyticus
3.2.1.94 60
-
 60% activity at pH 5 after 30 min Arthrobacter dextranolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.94 496
-
 Dextran 60°C, pH 3.5 Arthrobacter dextranolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.94 3.5
-
-
 Arthrobacter dextranolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.94 2.5 6 50% activity between pH 2.5 and pH 6 Arthrobacter dextranolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.94 3.5 10 enzyme is stable at pH 4-8 and stable at pH 3.5-10 with 50% of the original activity Arthrobacter dextranolyticus