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Literature summary extracted from

  • Lisboa De Marco, J.; Valadares-Inglis, M.C.; Felix, C.R.
    Purification and characterization of an N-acetylglucosaminidase produced by a Trichoderma harzianum strain which controls Crinipellis perniciosa (2004), Appl. Microbiol. Biotechnol., 64, 70-75.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 AlCl3 1 mM, 24% inhibition Trichoderma harzianum
3.2.1.52 CaCl2 1 mM, 30% inhibition Trichoderma harzianum
3.2.1.52 CoCl2 1 mM, complete inhibition Trichoderma harzianum
3.2.1.52 CuSO4 1 mM, 36% inhibition Trichoderma harzianum
3.2.1.52 FeCl3 1 mM, complete inhibition Trichoderma harzianum
3.2.1.52 glucose 1 mM, 35% inhibition Trichoderma harzianum
3.2.1.52 MnCl2 1 mM, complete inhibition Trichoderma harzianum
3.2.1.52 ZnSO4 1 mM, 77% inhibition Trichoderma harzianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.00806
-
 p-nitrophenyl-N-acetylglucosaminide pH 6.0, 37°C Trichoderma harzianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 36000
-
 x * 36000, SDS-PAGE Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Trichoderma harzianum
-
 isolate 1051
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52
-
 Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 p-nitrophenyl-N-acetylglucosaminide + H2O
-
 Trichoderma harzianum p-nitrophenol + N-acetylglucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 36000, SDS-PAGE Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 50 60
-
 Trichoderma harzianum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 30 60 30°C: about 55% of maximal activity, 50-60°C: maximal activity, 70°C: less than 10% of maximal activity Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 37
-
 30 min, about 30% loss of activity. About 40% loss of acticity after 12 h Trichoderma harzianum
3.2.1.52 50
-
 about 50% loss of activity after 1 h. About 85% loss of activity after 12 h Trichoderma harzianum
3.2.1.52 60
-
 complete loss of activity after 30 min Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4
-
 hydrolysis of p-nitrophenyl-N-acetylglucosaminide Trichoderma harzianum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 3 6 pH 3.0: about 55% of maximal activity, pH 6.0: about 70% of maximal activity, hydrolysis of p-nitrophenyl-N-acetylglucosaminide Trichoderma harzianum