EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.214 | synthesis | enzyme is useful for production of chiral alcohols | Candida parapsilosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.214 | isozyme CPR-C2, DNA and amino acid sequence determination and analysis, overexpression of the isozyme in Escherichia coli strain BL21(DE3), addition of IPTG highly decreases the expression rate of the isozyme | Candida parapsilosis |
1.1.1.214 | isozymes CPR-C1, DNA and amino acid sequence determination and analysis, overexpression of the isozyme in Escherichia coli strain BL21(DE3), addition of IPTG highly decreases the expression rate of the isozyme | Candida parapsilosis |
1.1.1.358 | expressed in Escherichia coli BL21(DE3) cells | Candida parapsilosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.214 | (R)-pantolactone | substrate inhibition of the recombinant isozyme expressed in Escherichia coli at a concentration above 10 mg/ml | Candida parapsilosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.214 | 36000 | - |
x * 36000, recombinant isozyme CPR-C2, SDS-PAGE | Candida parapsilosis |
1.1.1.214 | 38000 | - |
x * 38000, recombinant isozyme CPR-C1, SDS-PAGE | Candida parapsilosis |
1.1.1.358 | 34491 | - |
x * 34491, isoform CPR-C1, calculated from amino acid sequence | Candida parapsilosis |
1.1.1.358 | 34650 | - |
x * 34650, isoform CPR-C2, calculated from amino acid sequence | Candida parapsilosis |
1.1.1.358 | 36000 | - |
x * 36000, isoform CPR-C2, SDS-PAGE | Candida parapsilosis |
1.1.1.358 | 38000 | - |
x * 38000, isoform CPR-C1, SDS-PAGE | Candida parapsilosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.214 | (R)-pantolactone + NADP+ | Candida parapsilosis | - |
2-dehydropantolactone + NADPH | - |
? | |
1.1.1.214 | (R)-pantolactone + NADP+ | Candida parapsilosis IFO 0708 | - |
2-dehydropantolactone + NADPH | - |
? | |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ | Candida parapsilosis | - |
(R)-pantolactone + NADP+ | - |
? | |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ | Candida parapsilosis IFO 0708 | - |
(R)-pantolactone + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.214 | Candida parapsilosis | Q76L36 | isozyme CPR-C2; 2 isozymes CPR-C1 and CPR-C2 | - |
1.1.1.214 | Candida parapsilosis | Q76L37 | isozyme CPR-C1; 2 isozymes CPR-C1 and CPR-C2 | - |
1.1.1.214 | Candida parapsilosis IFO 0708 | Q76L36 | isozyme CPR-C2; 2 isozymes CPR-C1 and CPR-C2 | - |
1.1.1.214 | Candida parapsilosis IFO 0708 | Q76L37 | isozyme CPR-C1; 2 isozymes CPR-C1 and CPR-C2 | - |
1.1.1.358 | Candida parapsilosis | Q76L36 | - |
- |
1.1.1.358 | Candida parapsilosis | Q76L37 | - |
- |
1.1.1.358 | Candida parapsilosis IFO 0708 | Q76L36 | - |
- |
1.1.1.358 | Candida parapsilosis IFO 0708 | Q76L37 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.214 | 5.03 | - |
recombinant isozyme CPR-C2 in cell extract of expressing Escherichia coli cells in absence of IPTG | Candida parapsilosis |
1.1.1.214 | 19.3 | - |
recombinant isozyme CPR-C1 in cell extract of expressing Escherichia coli cells in absence of IPTG | Candida parapsilosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.214 | (R)-pantolactone + NADP+ | - |
Candida parapsilosis | 2-dehydropantolactone + NADPH | - |
? | |
1.1.1.214 | (R)-pantolactone + NADP+ | i.e. ketopantoyl lactone | Candida parapsilosis | 2-dehydropantolactone + NADPH | - |
? | |
1.1.1.214 | (R)-pantolactone + NADP+ | i.e. ketopantoyl lactone | Candida parapsilosis IFO 0708 | 2-dehydropantolactone + NADPH | - |
? | |
1.1.1.214 | (R)-pantolactone + NADP+ | - |
Candida parapsilosis IFO 0708 | 2-dehydropantolactone + NADPH | - |
? | |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ | - |
Candida parapsilosis | (R)-pantolactone + NADP+ | - |
? | |
1.1.1.358 | 2-dehydropantolactone + NADPH + H+ | - |
Candida parapsilosis IFO 0708 | (R)-pantolactone + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.214 | ? | x * 36000, recombinant isozyme CPR-C2, SDS-PAGE | Candida parapsilosis |
1.1.1.214 | ? | x * 38000, recombinant isozyme CPR-C1, SDS-PAGE | Candida parapsilosis |
1.1.1.358 | ? | x * 34491, isoform CPR-C1, calculated from amino acid sequence | Candida parapsilosis |
1.1.1.358 | ? | x * 34650, isoform CPR-C2, calculated from amino acid sequence | Candida parapsilosis |
1.1.1.358 | ? | x * 36000, isoform CPR-C2, SDS-PAGE | Candida parapsilosis |
1.1.1.358 | ? | x * 38000, isoform CPR-C1, SDS-PAGE | Candida parapsilosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.214 | CPR-C1 | - |
Candida parapsilosis |
1.1.1.214 | CPR-C2 | - |
Candida parapsilosis |
1.1.1.214 | ketopantoyl lactone reductase | - |
Candida parapsilosis |
1.1.1.358 | conjugated polyketone reductase | - |
Candida parapsilosis |
1.1.1.358 | CPR-C1 | isoform | Candida parapsilosis |
1.1.1.358 | CPR-C2 | isoform | Candida parapsilosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.214 | 28 | - |
assay at | Candida parapsilosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.214 | 9 | - |
about | Candida parapsilosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.214 | 4 | 9 | - |
Candida parapsilosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.214 | NADP+ | - |
Candida parapsilosis | |
1.1.1.358 | NADPH | - |
Candida parapsilosis |