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Literature summary extracted from

  • Kataoka, M.; Delacruz-Hidalgo, A.R.G.; Akond, M.A.; Sakuradani, E.; Kita, K.; Shimizu, S.
    Gene cloning and overexpression of two conjugated polyketone reductases, novel aldo-keto reductase family enzymes, of Candida parapsilosis. Investigation of hydroxamic acids as laccase-mediators for pulp bleaching (2004), Appl. Microbiol. Biotechnol., 64, 359-366.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.214 synthesis enzyme is useful for production of chiral alcohols Candida parapsilosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.214 isozyme CPR-C2, DNA and amino acid sequence determination and analysis, overexpression of the isozyme in Escherichia coli strain BL21(DE3), addition of IPTG highly decreases the expression rate of the isozyme Candida parapsilosis
1.1.1.214 isozymes CPR-C1, DNA and amino acid sequence determination and analysis, overexpression of the isozyme in Escherichia coli strain BL21(DE3), addition of IPTG highly decreases the expression rate of the isozyme Candida parapsilosis
1.1.1.358 expressed in Escherichia coli BL21(DE3) cells Candida parapsilosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.214 (R)-pantolactone substrate inhibition of the recombinant isozyme expressed in Escherichia coli at a concentration above 10 mg/ml Candida parapsilosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.214 36000
-
x * 36000, recombinant isozyme CPR-C2, SDS-PAGE Candida parapsilosis
1.1.1.214 38000
-
x * 38000, recombinant isozyme CPR-C1, SDS-PAGE Candida parapsilosis
1.1.1.358 34491
-
x * 34491, isoform CPR-C1, calculated from amino acid sequence Candida parapsilosis
1.1.1.358 34650
-
x * 34650, isoform CPR-C2, calculated from amino acid sequence Candida parapsilosis
1.1.1.358 36000
-
x * 36000, isoform CPR-C2, SDS-PAGE Candida parapsilosis
1.1.1.358 38000
-
x * 38000, isoform CPR-C1, SDS-PAGE Candida parapsilosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.214 (R)-pantolactone + NADP+ Candida parapsilosis
-
2-dehydropantolactone + NADPH
-
?
1.1.1.214 (R)-pantolactone + NADP+ Candida parapsilosis IFO 0708
-
2-dehydropantolactone + NADPH
-
?
1.1.1.358 2-dehydropantolactone + NADPH + H+ Candida parapsilosis
-
(R)-pantolactone + NADP+
-
?
1.1.1.358 2-dehydropantolactone + NADPH + H+ Candida parapsilosis IFO 0708
-
(R)-pantolactone + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.214 Candida parapsilosis Q76L36 isozyme CPR-C2; 2 isozymes CPR-C1 and CPR-C2
-
1.1.1.214 Candida parapsilosis Q76L37 isozyme CPR-C1; 2 isozymes CPR-C1 and CPR-C2
-
1.1.1.214 Candida parapsilosis IFO 0708 Q76L36 isozyme CPR-C2; 2 isozymes CPR-C1 and CPR-C2
-
1.1.1.214 Candida parapsilosis IFO 0708 Q76L37 isozyme CPR-C1; 2 isozymes CPR-C1 and CPR-C2
-
1.1.1.358 Candida parapsilosis Q76L36
-
-
1.1.1.358 Candida parapsilosis Q76L37
-
-
1.1.1.358 Candida parapsilosis IFO 0708 Q76L36
-
-
1.1.1.358 Candida parapsilosis IFO 0708 Q76L37
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.214 5.03
-
recombinant isozyme CPR-C2 in cell extract of expressing Escherichia coli cells in absence of IPTG Candida parapsilosis
1.1.1.214 19.3
-
recombinant isozyme CPR-C1 in cell extract of expressing Escherichia coli cells in absence of IPTG Candida parapsilosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.214 (R)-pantolactone + NADP+
-
Candida parapsilosis 2-dehydropantolactone + NADPH
-
?
1.1.1.214 (R)-pantolactone + NADP+ i.e. ketopantoyl lactone Candida parapsilosis 2-dehydropantolactone + NADPH
-
?
1.1.1.214 (R)-pantolactone + NADP+ i.e. ketopantoyl lactone Candida parapsilosis IFO 0708 2-dehydropantolactone + NADPH
-
?
1.1.1.214 (R)-pantolactone + NADP+
-
Candida parapsilosis IFO 0708 2-dehydropantolactone + NADPH
-
?
1.1.1.358 2-dehydropantolactone + NADPH + H+
-
Candida parapsilosis (R)-pantolactone + NADP+
-
?
1.1.1.358 2-dehydropantolactone + NADPH + H+
-
Candida parapsilosis IFO 0708 (R)-pantolactone + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.214 ? x * 36000, recombinant isozyme CPR-C2, SDS-PAGE Candida parapsilosis
1.1.1.214 ? x * 38000, recombinant isozyme CPR-C1, SDS-PAGE Candida parapsilosis
1.1.1.358 ? x * 34491, isoform CPR-C1, calculated from amino acid sequence Candida parapsilosis
1.1.1.358 ? x * 34650, isoform CPR-C2, calculated from amino acid sequence Candida parapsilosis
1.1.1.358 ? x * 36000, isoform CPR-C2, SDS-PAGE Candida parapsilosis
1.1.1.358 ? x * 38000, isoform CPR-C1, SDS-PAGE Candida parapsilosis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.214 CPR-C1
-
Candida parapsilosis
1.1.1.214 CPR-C2
-
Candida parapsilosis
1.1.1.214 ketopantoyl lactone reductase
-
Candida parapsilosis
1.1.1.358 conjugated polyketone reductase
-
Candida parapsilosis
1.1.1.358 CPR-C1 isoform Candida parapsilosis
1.1.1.358 CPR-C2 isoform Candida parapsilosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.214 28
-
assay at Candida parapsilosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.214 9
-
about Candida parapsilosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.214 4 9
-
Candida parapsilosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.214 NADP+
-
Candida parapsilosis
1.1.1.358 NADPH
-
Candida parapsilosis