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Literature summary extracted from

  • Baldrian, P.
    Purification and characterization of laccase from the white-rot fungus Daedalea quercina and decolorization of synthetic dyes by the enzyme (2004), Appl. Microbiol. Biotechnol., 63, 560-563.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Cd2+ 1 mM, 28% of inhibition Daedalea quercina
1.10.3.2 Co2+ 1 mM, 51% of inhibition Daedalea quercina
1.10.3.2 dithiothreitol 0.1 mM, complete inhibition Daedalea quercina
1.10.3.2 EDTA 1 mM, 90% residual activity Daedalea quercina
1.10.3.2 glutathione 1 mM, complete inhibition Daedalea quercina
1.10.3.2 Hg2+ 1 mM, 93% of inhibition Daedalea quercina
1.10.3.2 kojic acid 1 mM, 57% residual activity Daedalea quercina
1.10.3.2 L-cysteine 0.1 mM, complete inhibition Daedalea quercina
1.10.3.2 Mn2+ 1 mM, 91% of inhibition Daedalea quercina
1.10.3.2 Sodium azide 0.1 mM, complete inhibition Daedalea quercina
1.10.3.2 Thiourea 1 mM, complete inhibition Daedalea quercina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.038
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, 22°C Daedalea quercina
1.10.3.2 0.048
-
 2,6-dimethoxyphenol pH 5.0, 22°C Daedalea quercina
1.10.3.2 0.093
-
 guaiacol pH 5.0, 22°C Daedalea quercina
1.10.3.2 0.131
-
 syringaldehyde pH 5.0, 22°C Daedalea quercina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper 10 mM increase activity by 17%, 0.1 mM increase activity by 100% Daedalea quercina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 69000
-
 1 * 69000, SDS-PAGE Daedalea quercina
1.10.3.2 71000
-
 gel filtration Daedalea quercina

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Daedalea quercina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Daedalea quercina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Daedalea quercina ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Daedalea quercina ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Daedalea quercina ? + H2O
-
 ?
1.10.3.2 syringaldehyde + O2
-
 Daedalea quercina ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 69000, SDS-PAGE Daedalea quercina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 60
-
 at pH 3.0 Daedalea quercina
1.10.3.2 70
-
 at pH 5.0 Daedalea quercina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 45
-
 stable up to Daedalea quercina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2
-
 or below, substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Daedalea quercina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 2.5
-
 33% loss of activity after 24h Daedalea quercina
1.10.3.2 4
-
 15% loss of activity after 24h Daedalea quercina
1.10.3.2 6
-
 less than 3% loss of activity after 24h Daedalea quercina

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Daedalea quercina isoelectric focusing
-
 3