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Literature summary extracted from

  • Xiao, Y.Z.; Tu, X.M.; Wang, J.; Zhang, M.; Cheng, Q.; Zeng, W.Y.; Shi, Y.Y.
    Purification, molecular characterization and reactivity with aromatic compounds of a laccase from basidiomycete Trametes sp. strain AH28-2 (2003), Appl. Microbiol. Biotechnol., 60, 700-707.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 cyanide 0.1 mM, strong Trametes sp.
1.10.3.2 Sodium azide 0.1 mM, strong Trametes sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.025
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 25°C Trametes sp.
1.10.3.2 0.0255
-
 2,6-dimethoxyphenol pH 4.5, 25°C Trametes sp.
1.10.3.2 0.42
-
 guaiacol pH 4.5, 25°C Trametes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 57000
-
 gel filtration Trametes sp.
1.10.3.2 58500
-
 1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry Trametes sp.
1.10.3.2 62000
-
 1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry Trametes sp.

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes sp. Q5I7J0 two isozymes after induction by kraft lignin, laccase as forms 85% of total activity
-
1.10.3.2 Trametes sp. AH28-2 Q5I7J0 two isozymes after induction by kraft lignin, laccase as forms 85% of total activity
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein carbohydrate content 11-12% Trametes sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 isozyme laccase Trametes sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture supernatant
-
 Trametes sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 66.8
-
 pH 4.5, 25°C Trametes sp.
1.10.3.2 79
-
 pH 4.5, 25°C Trametes sp.
1.10.3.2 670
-
 pH 4.5, 25°C Trametes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sp. ? + H2O
-
 ?
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
1.10.3.2 2,6-dichlorophenol + O2
-
 Trametes sp. ? + H2O
-
 ?
1.10.3.2 2,6-dichlorophenol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes sp. ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
1.10.3.2 fluorene + O2
-
 Trametes sp. ? + H2O
-
 ?
1.10.3.2 fluorene + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes sp. ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry Trametes sp.

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 laccase A
-
 Trametes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
 substrate guaiacol Trametes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 75
-
 27 min, 50% residual activity Trametes sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 69
-
 guaiacol pH 4.5, 25°C Trametes sp.
1.10.3.2 81
-
 2,6-dimethoxyphenol pH 4.5, 25°C Trametes sp.
1.10.3.2 692
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 25°C Trametes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4.5
-
 substrate guaiacol Trametes sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4.2 8 good stability Trametes sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Trametes sp. isoelectric focusing
-
 4.2