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Literature summary extracted from
- The mannitol dehydrogenase gene (mdh) from Leuconostoc mesenteroides is distinct from other known bacterial mdh genes (2002), Appl. Microbiol. Biotechnol., 59, 665-671.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.67 | fusion of six His codons to the 3'-end of the mdh gene and expression in Escherichia coli M15. The enzyme shares significant sequence similarity with the medium-chain dehydrogenase/reductase protein family | Leuconostoc mesenteroides |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.67 | PCMB | 0.1 mM, complete inhibition | Leuconostoc mesenteroides |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.67 | 32 | - |
D-mannitol | pH 8.6 | Leuconostoc mesenteroides | |
| 1.1.1.67 | 71 | - |
D-fructose | pH 5.3 | Leuconostoc mesenteroides | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.67 | 41000 | - |
1 * 41000, SDS-PAGE | Leuconostoc mesenteroides |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.67 | Leuconostoc mesenteroides | Q8KQG6 | ATCC-9135 | - |
| 1.1.1.67 | Leuconostoc mesenteroides ATCC -9135 | Q8KQG6 | ATCC-9135 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.67 | recombinant enzyme | Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.67 | D-fructose 1-phosphate + NADH | - |
Leuconostoc mesenteroides | ? | - |
? | |
| 1.1.1.67 | D-fructose 1-phosphate + NADH | - |
Leuconostoc mesenteroides ATCC -9135 | ? | - |
? | |
| 1.1.1.67 | D-mannitol + NAD+ | - |
Leuconostoc mesenteroides | D-fructose + NADH + H+ | - |
r | |
| 1.1.1.67 | D-mannitol + NAD+ | - |
Leuconostoc mesenteroides ATCC -9135 | D-fructose + NADH + H+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.67 | monomer | 1 * 41000, SDS-PAGE | Leuconostoc mesenteroides |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.67 | MDH | - |
Leuconostoc mesenteroides |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.67 | NAD+ | - |
Leuconostoc mesenteroides | |
| 1.1.1.67 | NADH | - |
Leuconostoc mesenteroides | |
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Release 2023.1 (January 2023)
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