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Literature summary extracted from

  • Fayad, K.P.; Simao-Beaunoir, A.M.; Gauthier, A.; Leclerc, C.; Mamady, H.; Beaulieu, C.; Brzezinski, R.
    Purification and properties of a beta-1,6-glucanase from Streptomyces sp. EF-14, an actinomycete antagonistic to Phytophthora spp (2001), Appl. Microbiol. Biotechnol., 57, 117-123.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.75 additional information no inhibitory: D-glucono-1,5-lactone at 1-20 mM Streptomyces sp. EF-14

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.75 65000
-
 gel filtration Streptomyces sp. EF-14

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.75 Streptomyces sp. EF-14
-
 growth medium with lyophilized cells of Candida utilis as mian nutrient source
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.75
-
 Streptomyces sp. EF-14

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.75 culture supernatant
-
 Streptomyces sp. EF-14
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.75 chitosan + H2O 3% of the activity with pustulan Streptomyces sp. EF-14 ?
-
 ?
3.2.1.75 additional information no substrate: laminarin Streptomyces sp. EF-14 ?
-
 ?
3.2.1.75 pustulan + H2O
-
 Streptomyces sp. EF-14 gentobiose + D-glucose + ?
-
 ?
3.2.1.75 starch + H2O 8% of the activity with pustulan Streptomyces sp. EF-14 ?
-
 ?
3.2.1.75 xylan + H2O 12% of the activity with pustulan Streptomyces sp. EF-14 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.75 monomer 1 * 65000, SDS-PAGE Streptomyces sp. EF-14
3.2.1.75 More N-terminal amino acid sequence Streptomyces sp. EF-14

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.75 55
-
-
 Streptomyces sp. EF-14

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.75 45
-
 unstable above, presence of pustulan stabilizes Streptomyces sp. EF-14

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.75 5.5
-
-
 Streptomyces sp. EF-14

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.75 4.5 6.5 at least 80% of maximum activity in this range Streptomyces sp. EF-14

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.75 Streptomyces sp. EF-14 isoelectric focusing
-
 5.5