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Literature summary extracted from

  • Heydari, M.; Ohshima, T.; Nunoura-Kominato, N.; Sakuraba, H.
    Highly stable L-lysine 6-dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization, gene cloning and sequencing, and expression (2004), Appl. Environ. Microbiol., 70, 937-942.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.18 expression in Escherichia coli Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.18 HgCl2 1 mM, 85% inhibition Geobacillus stearothermophilus
1.4.1.18 p-chloromercuribenzoate 1 mM, 33% inhibition Geobacillus stearothermophilus
1.4.1.18 Pb(acetate)2 1 mM, 37% inhibition Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.18 0.088
-
 NAD+ 50°C, pH 10.0 Geobacillus stearothermophilus
1.4.1.18 0.48
-
 NADP+ 50°C, pH 10.0 Geobacillus stearothermophilus
1.4.1.18 0.73
-
 L-lysine 50°C, pH 10.0 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.18 42239
-
 x * 42239, deduced from nucleotide sequence Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.18 L-lysine + NAD+ + H2O Geobacillus stearothermophilus
-
 2-aminoadipate-6-semialdehyde + NADH + H+ + NH3 cyclizes nonenzymatically to DELTA1-piperidine-6-carboxylate ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.18 Geobacillus stearothermophilus Q9AJC6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.18 native LysDH, SuperQ-Toyopearl, Red-Sepharose, Gigapite, Cellulofine, recombinant LysDH, Red-Sepharose Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.18 6.2
-
 native LysDH Geobacillus stearothermophilus
1.4.1.18 7.8
-
 recombinant LysDH Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
1.4.1.18 4°C, more than 3 months, no loss of activity Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.18 L-lysine + NAD+ + H2O
-
 Geobacillus stearothermophilus 2-aminoadipate-6-semialdehyde + NADH + H+ + NH3 cyclizes nonenzymatically to DELTA1-piperidine-6-carboxylate ?
1.4.1.18 L-lysine + NADP+ 22% of activity with NAD+ Geobacillus stearothermophilus 2-aminoadipate-6-semialdehyde + NADPH cyclizes nonenzymatically to DELTA1-piperidine-6-carboxylate ?
1.4.1.18 S-(beta-aminoethyl)-L-cysteine + NAD+ 11% of activity with L-lysine Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.18 ? x * 42239, deduced from nucleotide sequence Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.18 LysDH
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.18 70
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.18 60
-
 no loss of activity after 10 min at pH 7.2 Geobacillus stearothermophilus
1.4.1.18 65
-
 approx. 25% loss of activity after 10 min at pH 7.2 in the absence of 5 mM lysine, no loss of activity in the presence of 5 mM lysine Geobacillus stearothermophilus
1.4.1.18 70
-
 approx. 55% loss of activity after 10 min at pH 7.2 Geobacillus stearothermophilus
1.4.1.18 80
-
 complete loss of activity after 10 min at pH 7.2 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.18 10
-
 native LysDH Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.18 7 10.8 approx. 35% of maximal activity at pH 7.0, approx. 50% of maximal activity at pH 10.5 Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.18 6 9 no loss of activity after 30 min at 50°C Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.18 NAD+
-
 Geobacillus stearothermophilus