EC Number | Cloned (Comment) | Organism |
---|---|---|
1.12.1.2 | DNA and amino acid sequence determination and analysis, HoxE is encoded in a cluster of 5 genes encoding the enzyme complex subunits, sigma54-type promotor with probably inducible expression of the operon | Thiocapsa roseopersicina |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.12.1.2 | additional information | in-frame deletion of HoxE, deletion of HoxH | Thiocapsa roseopersicina |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.1.2 | cytoplasm | - |
Thiocapsa roseopersicina | 5737 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD+ | Thiocapsa roseopersicina | in vivo H2 evolution under nitrogenase-repressed conditions | H+ + NADH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.1.2 | Thiocapsa roseopersicina | Q6XQK5 | purple sulfur photosynthetic bacterium | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.1.2 | partially | Thiocapsa roseopersicina |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | additional information | - |
determination of H2 production rates in vivo | Thiocapsa roseopersicina |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD+ | - |
Thiocapsa roseopersicina | H+ + NADH | - |
r | |
1.12.1.2 | H2 + NAD+ | in vivo H2 evolution under nitrogenase-repressed conditions | Thiocapsa roseopersicina | H+ + NADH | - |
r | |
1.12.1.2 | H2 + oxidized methyl viologen | - |
Thiocapsa roseopersicina | H+ + reduced methyl viologen | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.1.2 | More | the subunit HoxE, which is essential for the bidirectional hydrogenase activity, is included in a pentameric bidirectional hydrogenase complex HoxEFUYH of the cyanobacterial-type, complex components are HoxE, HoxF, HoxH, HoxU, and HoxY | Thiocapsa roseopersicina |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.1.2 | Hox | - |
Thiocapsa roseopersicina |
1.12.1.2 | NAD+-reducing NiFe hydrogenase | - |
Thiocapsa roseopersicina |
1.12.1.2 | NiFe hydrogenase | - |
Thiocapsa roseopersicina |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 7 | - |
assay at, H2 evolution | Thiocapsa roseopersicina |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.2 | NAD+ | - |
Thiocapsa roseopersicina | |
1.12.1.2 | NADH | - |
Thiocapsa roseopersicina |