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Literature summary extracted from

  • Sauer, M.; Branduardi, P.; Valli, M.; Porro, D.
    Production of L-ascorbic acid by metabolically engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii (2004), Appl. Environ. Microbiol., 70, 6086-6091.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.116 synthesis production of L-ascorbic acid and secretion into culture medium by overexpression of enzyme and arabinone-1,4-lactone oxidase in Saccharomyces cerevisiae and Zygosaccharomyces bailii Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.116 gene ARA1, co-overexpression of the enzyme and the D-arabinono-1,4-lactone oxidase and/or L-galactono-1,4-lactone dehydrogenase in Saccharomyces cerevisiae strains result in overproduction and accumulation of vitamin C in the culture medium, while overexpression of the D-arabinose dehydrogenase alone does not alter the L-ascorbate content, overview Saccharomyces cerevisiae
1.1.3.37 yeasts do not possess an endogenous biochemical pathway for the synthesis of vitamin C. Overexpression of D-arabinose dehydrogenase and D-arabino-1,4-lactone oxidase enhance this ability significantly, production of vitamin C from metabiolically engineered Saccharomyces cerevisaie as well as from Zygosaccharomyces bailii Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.116 D-arabinose + NAD+ Saccharomyces cerevisiae
-
 D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.116 D-arabinose + NAD+ Saccharomyces cerevisiae GRF18U
-
 D-arabinono-1,4-lactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.116 Saccharomyces cerevisiae
-
 recombinant enzyme overexpressed in Saccharomyces cerevisiae and Zygosaccharomyces bailii
-
1.1.1.116 Saccharomyces cerevisiae
-
 gene ARA1
-
1.1.1.116 Saccharomyces cerevisiae GRF18U
-
 gene ARA1
-
1.1.3.37 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.116 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.116 D-arabinose + NAD+
-
 Saccharomyces cerevisiae D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.116 D-arabinose + NAD+
-
 Saccharomyces cerevisiae GRF18U D-arabinono-1,4-lactone + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.116 ARA
-
 Saccharomyces cerevisiae
1.1.1.116 D-arabinose dehydrogenase
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.116 NAD+
-
 Saccharomyces cerevisiae