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Literature summary extracted from

  • Ait-Lahsen, H.; Soler, A.; Rey, M.; de La Cruz, J.; Monte, E.; Llobell, A.
    An antifungal exo-alpha-1,3-glucanase (AGN13.1) from the biocontrol fungus Trichoderma harzianum (2001), Appl. Environ. Microbiol., 67, 5833-5839.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.84 biotechnology transgenic plants might have improved resistance to fungal pathogens Trichoderma harzianum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.84
-
 Trichoderma harzianum

General Stability

EC Number General Stability Organism
3.2.1.84 alpha-1,3-glucan stabilizes the enzyme Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.84 extracellular
-
 Trichoderma harzianum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.84 63800
-
 calculated from amino acid sequence Trichoderma harzianum
3.2.1.84 67000
-
 gel filtration Trichoderma harzianum
3.2.1.84 72000
-
 SDS-PAGE Trichoderma harzianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.84 additional information Trichoderma harzianum lytic and antifungal activity against fungal plant panthogens ?
-
 ?
3.2.1.84 additional information Trichoderma harzianum CECT2413 lytic and antifungal activity against fungal plant panthogens ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.84 Trichoderma harzianum
-
 sequence was found to be identical to previously published data
-
3.2.1.84 Trichoderma harzianum CECT2413
-
 sequence was found to be identical to previously published data
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.84
-
 Trichoderma harzianum

Storage Stability

EC Number Storage Stability Organism
3.2.1.84 -20°C, 100 mM acetate buffer, pH 5.5 Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.84 alpha-1,3-glucan + H2O
-
 Trichoderma harzianum alpha-D-glucose
-
 ?
3.2.1.84 alpha-1,3-glucan + H2O
-
 Trichoderma harzianum CECT2413 alpha-D-glucose
-
 ?
3.2.1.84 alpha-1,3-mutan + H2O
-
 Trichoderma harzianum alpha-D-glucose
-
 ?
3.2.1.84 alpha-1,3-mutan + H2O
-
 Trichoderma harzianum CECT2413 alpha-D-glucose
-
 ?
3.2.1.84 beta-glucan:chitin + H2O different activities with substrates from cell walls of different microorganisms Trichoderma harzianum ?
-
 ?
3.2.1.84 beta-glucan:chitin + H2O different activities with substrates from cell walls of different microorganisms Trichoderma harzianum CECT2413 ?
-
 ?
3.2.1.84 additional information lytic and antifungal activity against fungal plant panthogens Trichoderma harzianum ?
-
 ?
3.2.1.84 additional information no activity with beta-glucan or alpha-1,4 or alpha-1,6 linked glucose chains Trichoderma harzianum ?
-
 ?
3.2.1.84 additional information lytic and antifungal activity against fungal plant panthogens Trichoderma harzianum CECT2413 ?
-
 ?
3.2.1.84 additional information no activity with beta-glucan or alpha-1,4 or alpha-1,6 linked glucose chains Trichoderma harzianum CECT2413 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.84 monomer equal molecular weight determined with SDS-PAGE (72000 Da) and gel filtration (67000 Da) Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.84 55
-
-
 Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.84 5.5
-
-
 Trichoderma harzianum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.84 Trichoderma harzianum isoelectric focusing
-
 7.1
3.2.1.84 Trichoderma harzianum basic chromatofocusing
-
 7.5