Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Manzanares, P.; van den Broeck, H.C.; de Graaff, L.H.; Visser, J.
    Purification and characterization of two different alpha-L-rhamnosidases, RhaA and RhaB, from Aspergillus aculeatus (2001), Appl. Environ. Microbiol., 67, 2230-2234.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 L-rhamnose competitive Aspergillus aculeatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 0.3
-
 p-nitrophenyl-alpha-L-rhamnopyranoside pH 4.5, 30°C Aspergillus aculeatus
3.2.1.40 2.8
-
 p-nitrophenyl-alpha-L-rhamnopyranoside pH 4.5, 30°C Aspergillus aculeatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.40 extracellular growth on hesperidin Aspergillus aculeatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 85000
-
 x * 85000, SDS-PAGE Aspergillus aculeatus
3.2.1.40 92000
-
 x * 92000, SDS-PAGE Aspergillus aculeatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Aspergillus aculeatus Q9C1M9 isozyme RhaA, groth on hesperidin
-
3.2.1.40 Aspergillus aculeatus Q9HFW5 isozyme RhaB, growth on hesperidin
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.40 glycoprotein only N-glycosylation, about 15% carbohydrate Aspergillus aculeatus
3.2.1.40 glycoprotein only N-glycosylation, about 24% carbohydrate Aspergillus aculeatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Aspergillus aculeatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 13
-
 pH 4.5, 30°C Aspergillus aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 hesperidin + H2O
-
 Aspergillus aculeatus L-rhamnose + ?
-
 ?
3.2.1.40 additional information no substrate: quercitrin Aspergillus aculeatus ?
-
 ?
3.2.1.40 naringin + H2O
-
 Aspergillus aculeatus L-rhamnose + ?
-
 ?
3.2.1.40 p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Aspergillus aculeatus p-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 rutin + H2O
-
 Aspergillus aculeatus L-rhamnose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 ? x * 85000, SDS-PAGE Aspergillus aculeatus
3.2.1.40 ? x * 92000, SDS-PAGE Aspergillus aculeatus
3.2.1.40 More N-terminal amino acid sequence Aspergillus aculeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 30
-
 20 h, 80% residual stability Aspergillus aculeatus
3.2.1.40 30
-
 20 h, 90% residual stability Aspergillus aculeatus
3.2.1.40 40
-
 4 h, stable Aspergillus aculeatus
3.2.1.40 55
-
 4 h, 75% residual stability Aspergillus aculeatus
3.2.1.40 55
-
 4 h, 87% residual stability Aspergillus aculeatus
3.2.1.40 65
-
 4 h, 55% residual stability Aspergillus aculeatus
3.2.1.40 65
-
 4 h, 60% residual stability Aspergillus aculeatus
3.2.1.40 75
-
 4 h, 50% residual stability Aspergillus aculeatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 4.5 5
-
 Aspergillus aculeatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.40 4.5 5
-
 Aspergillus aculeatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.40 3 5
-
 Aspergillus aculeatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.40 1.5
-
 L-rhamnose pH 4.5, 30°C Aspergillus aculeatus
3.2.1.40 4.2
-
 L-rhamnose pH 4.5, 30°C Aspergillus aculeatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.40 Aspergillus aculeatus shows microheterogenity upon isoelectric focusing
-
 additional information
3.2.1.40 Aspergillus aculeatus calculated from amino acid sequence
-
 6
3.2.1.40 Aspergillus aculeatus isoelectric focusing
-
 6