EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.1 | expression in Escherichia coli | Brucella anthropi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.1 | 1,10-phenanthroline | 1 mM, 23% inhibition | Brucella anthropi | |
3.2.2.1 | 8-hydroxyquinoline | 1 mM, 93% inhibition | Brucella anthropi | |
3.2.2.1 | Ag+ | 1 mM, 68% inhibition | Brucella anthropi | |
3.2.2.1 | Cu2+ | 1 mM, 57% inhibition | Brucella anthropi | |
3.2.2.1 | cytidine | 0.5 mM, 81% inhibition of activity with adenosine, 92% inhibition of activity with inosine, 91% inhibition of the activity with xanthosine, 94% inhibition of activity with guanosine | Brucella anthropi | |
3.2.2.1 | EDTA | 1 mM, 46% inhibition | Brucella anthropi | |
3.2.2.1 | Hg2+ | 1 mM,61% inhibition | Brucella anthropi | |
3.2.2.1 | Mn2+ | 1 mM, 34% inhibition | Brucella anthropi | |
3.2.2.1 | NaAsO2 | - |
Brucella anthropi | |
3.2.2.1 | NaCN | - |
Brucella anthropi | |
3.2.2.1 | NaF | - |
Brucella anthropi | |
3.2.2.1 | NaN3 | - |
Brucella anthropi | |
3.2.2.1 | uridine | 0.5 mM, 38% inhibition of activity with adenosine, 45% inhibition of activity with inosine, 35% inhibition of the activity with xanthosine, 58% inhibition of activity with guanosine | Brucella anthropi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.1 | 0.0118 | - |
adenosine | - |
Brucella anthropi | |
3.2.2.1 | 0.0158 | - |
Xanthosine | - |
Brucella anthropi | |
3.2.2.1 | 0.0395 | - |
Inosine | - |
Brucella anthropi | |
3.2.2.1 | 0.0563 | - |
guanosine | - |
Brucella anthropi |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.1 | Ca2+ | 1 mM, slightly enhances activity to 120% | Brucella anthropi | |
3.2.2.1 | Co2+ | 3 mM, reactivation after preincubation with 8-hydroxyquinoline | Brucella anthropi | |
3.2.2.1 | Zn2+ | 3 mM, reactivation after preincubation with 8-hydroxyquinoline | Brucella anthropi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 40000 | - |
4 * 40000, SDS-PAGE | Brucella anthropi |
3.2.2.1 | 172000 | - |
gel filtration | Brucella anthropi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.1 | Brucella anthropi | E15229 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.1 | - |
Brucella anthropi |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 90.3 | - |
- |
Brucella anthropi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.1 | 3'-AMP + H2O | 0.67% of the activity with adenosine | Brucella anthropi | adenine + D-ribose 3-phosphate | - |
? | |
3.2.2.1 | adenosine + H2O | - |
Brucella anthropi | adenine + D-ribose | - |
? | |
3.2.2.1 | guanosine + H2O | 29.5% of the activity with adenosine | Brucella anthropi | guanine + D-ribose | - |
? | |
3.2.2.1 | inosine + H2O | 78.9% of the activity with adenosine | Brucella anthropi | hypoxanthine + D-ribose | - |
? | |
3.2.2.1 | xanthosine + H2O | 63.5% of the activity with adenosine | Brucella anthropi | xanthine + D-ribose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.1 | tetramer | 4 * 40000, SDS-PAGE | Brucella anthropi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 50 | - |
- |
Brucella anthropi |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 40 | - |
pH 6.0, 30 min, stable up to | Brucella anthropi |
3.2.2.1 | 50 | - |
pH 6.0, 30 min, 50% loss of activity | Brucella anthropi |
3.2.2.1 | 60 | - |
pH 6.0, 30 min, 80% loss of activity | Brucella anthropi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 4.5 | - |
hydrolysis of guanosine | Brucella anthropi |
3.2.2.1 | 5.5 | - |
hydrolysis of inosine | Brucella anthropi |
3.2.2.1 | 6.5 | - |
hydrolysis of adenosine | Brucella anthropi |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 3.5 | 9 | hydrolysis of purine nucleosides | Brucella anthropi |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 4 | - |
30°C, 30 min, retains 40% of the initial activity | Brucella anthropi |
3.2.2.1 | 6 | 8.5 | 30°C, 30 min, enzyme retains more than 80% of the initial activity | Brucella anthropi |
3.2.2.1 | 9 | - |
30°C, 30 min, retains 40% of the initial activity | Brucella anthropi |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.1 | 0.00211 | - |
cytidine | pH 7.0, 30°C | Brucella anthropi | |
3.2.2.1 | 0.00415 | - |
cytidine | pH 7.0, 30°C | Brucella anthropi | |
3.2.2.1 | 0.0112 | - |
cytidine | pH 7.0, 30°C | Brucella anthropi | |
3.2.2.1 | 0.016 | - |
cytidine | pH 7.0, 30°C | Brucella anthropi |