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Literature summary extracted from

  • Ogawa, J.; Takeda, S.; Xie, S.X.; Hatanaka, H.; Ashikari, T.; Amachi, T.; Shimizu, S.
    Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi (2001), Appl. Environ. Microbiol., 67, 1783-1787.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.1 expression in Escherichia coli Brucella anthropi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.1 1,10-phenanthroline 1 mM, 23% inhibition Brucella anthropi
3.2.2.1 8-hydroxyquinoline 1 mM, 93% inhibition Brucella anthropi
3.2.2.1 Ag+ 1 mM, 68% inhibition Brucella anthropi
3.2.2.1 Cu2+ 1 mM, 57% inhibition Brucella anthropi
3.2.2.1 cytidine 0.5 mM, 81% inhibition of activity with adenosine, 92% inhibition of activity with inosine, 91% inhibition of the activity with xanthosine, 94% inhibition of activity with guanosine Brucella anthropi
3.2.2.1 EDTA 1 mM, 46% inhibition Brucella anthropi
3.2.2.1 Hg2+ 1 mM,61% inhibition Brucella anthropi
3.2.2.1 Mn2+ 1 mM, 34% inhibition Brucella anthropi
3.2.2.1 NaAsO2
-
Brucella anthropi
3.2.2.1 NaCN
-
Brucella anthropi
3.2.2.1 NaF
-
Brucella anthropi
3.2.2.1 NaN3
-
Brucella anthropi
3.2.2.1 uridine 0.5 mM, 38% inhibition of activity with adenosine, 45% inhibition of activity with inosine, 35% inhibition of the activity with xanthosine, 58% inhibition of activity with guanosine Brucella anthropi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.1 0.0118
-
adenosine
-
Brucella anthropi
3.2.2.1 0.0158
-
Xanthosine
-
Brucella anthropi
3.2.2.1 0.0395
-
Inosine
-
Brucella anthropi
3.2.2.1 0.0563
-
guanosine
-
Brucella anthropi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.1 Ca2+ 1 mM, slightly enhances activity to 120% Brucella anthropi
3.2.2.1 Co2+ 3 mM, reactivation after preincubation with 8-hydroxyquinoline Brucella anthropi
3.2.2.1 Zn2+ 3 mM, reactivation after preincubation with 8-hydroxyquinoline Brucella anthropi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.1 40000
-
4 * 40000, SDS-PAGE Brucella anthropi
3.2.2.1 172000
-
gel filtration Brucella anthropi

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Brucella anthropi E15229
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1
-
Brucella anthropi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.1 90.3
-
-
Brucella anthropi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 3'-AMP + H2O 0.67% of the activity with adenosine Brucella anthropi adenine + D-ribose 3-phosphate
-
?
3.2.2.1 adenosine + H2O
-
Brucella anthropi adenine + D-ribose
-
?
3.2.2.1 guanosine + H2O 29.5% of the activity with adenosine Brucella anthropi guanine + D-ribose
-
?
3.2.2.1 inosine + H2O 78.9% of the activity with adenosine Brucella anthropi hypoxanthine + D-ribose
-
?
3.2.2.1 xanthosine + H2O 63.5% of the activity with adenosine Brucella anthropi xanthine + D-ribose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.2.1 tetramer 4 * 40000, SDS-PAGE Brucella anthropi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.1 50
-
-
Brucella anthropi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.1 40
-
pH 6.0, 30 min, stable up to Brucella anthropi
3.2.2.1 50
-
pH 6.0, 30 min, 50% loss of activity Brucella anthropi
3.2.2.1 60
-
pH 6.0, 30 min, 80% loss of activity Brucella anthropi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.1 4.5
-
hydrolysis of guanosine Brucella anthropi
3.2.2.1 5.5
-
hydrolysis of inosine Brucella anthropi
3.2.2.1 6.5
-
hydrolysis of adenosine Brucella anthropi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.1 3.5 9 hydrolysis of purine nucleosides Brucella anthropi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.2.1 4
-
30°C, 30 min, retains 40% of the initial activity Brucella anthropi
3.2.2.1 6 8.5 30°C, 30 min, enzyme retains more than 80% of the initial activity Brucella anthropi
3.2.2.1 9
-
30°C, 30 min, retains 40% of the initial activity Brucella anthropi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.2.1 0.00211
-
cytidine pH 7.0, 30°C Brucella anthropi
3.2.2.1 0.00415
-
cytidine pH 7.0, 30°C Brucella anthropi
3.2.2.1 0.0112
-
cytidine pH 7.0, 30°C Brucella anthropi
3.2.2.1 0.016
-
cytidine pH 7.0, 30°C Brucella anthropi