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Literature summary extracted from

  • Hoyoux, A.; Jennes, I.; Dubois, P.; Genicot, S.; Dubail, F.; Francois, J.M.; Baise, E.; Feller, G.; Gerday, C.
    Cold-adapted beta-galactosidase from the antarctic psychrophile Pseudoalteromonas haloplanktis (2001), Appl. Environ. Microbiol., 67, 1529-1535.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.23 nutrition cold-adapted beta-galactosidase could be used to hydrolyze lactose in dairy products processed in refrigerated plants Pseudoalteromonas haloplanktis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expression in Escherichia coli Pseudoalteromonas haloplanktis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cu2+ 1 mM, strong inhibition Pseudoalteromonas haloplanktis
3.2.1.23 EDTA 5 mM, 90% loss of activity. Excess of Mg2+, Mn2+, Li2+ and Ca2+ restores activity Pseudoalteromonas haloplanktis
3.2.1.23 Ni2+ 1 mM, strong inhibition Pseudoalteromonas haloplanktis
3.2.1.23 Zn2+ 1 mM, strong inhibition Pseudoalteromonas haloplanktis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 2.4
-
 lactose 25°C Pseudoalteromonas haloplanktis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 118000
-
 x * 118000, SDS-PAGE Pseudoalteromonas haloplanktis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Pseudoalteromonas haloplanktis P81650 TAE 79
-
3.2.1.23 Pseudoalteromonas haloplanktis TAE 79 P81650 TAE 79
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Pseudoalteromonas haloplanktis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 138.2
-
-
 Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Pseudoalteromonas haloplanktis D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Pseudoalteromonas haloplanktis TAE 79 D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pseudoalteromonas haloplanktis o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pseudoalteromonas haloplanktis TAE 79 o-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 ? x * 118000, SDS-PAGE Pseudoalteromonas haloplanktis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 48
-
 hydrolysis of o-nitrophenyl beta-D-galactopyranoside Pseudoalteromonas haloplanktis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 28 48 28°C: about 45% of maximal activity, 48°C: optimum, 50°C: about 5% of maximal activity, hydrolysis of o-nitrophenyl beta-D-galactopyranoside Pseudoalteromonas haloplanktis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 48
-
 melting point of wild-type enzyme Pseudoalteromonas haloplanktis
3.2.1.23 50
-
 melting point of recombinant enzyme Pseudoalteromonas haloplanktis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 33
-
 lactose 25°C Pseudoalteromonas haloplanktis
3.2.1.23 203
-
 o-nitrophenyl beta-D-galactopyranoside 20°C Pseudoalteromonas haloplanktis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 8.5
-
 hydrolysis of o-nitrophenyl beta-D-galactopyranoside Pseudoalteromonas haloplanktis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 6.5 10 stable Pseudoalteromonas haloplanktis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.23 Pseudoalteromonas haloplanktis isoelectric focusing
-
 6.9