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Literature summary extracted from

  • Stoll, D.; Stalbrand, H.; Warren, R.A.J.
    Mannan-degrading enzymes from Cellulumonas fimi (1999), Appl. Environ. Microbiol., 65, 2598-2605.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.25 man2A gene is subcloned into the expression vector pET28A(+), yielding the plasmid pET28Man. The protein encoded by this plasmid carries a C-terminal His6 tag for purification, expression in Escherichia coli BL21 Cellulomonas fimi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.25 p-nitrophenyl-beta-mannoside above 0.4 mM Cellulomonas fimi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 additional information
-
 additional information as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the KM-value of 0.3 mM is only an estimate Cellulomonas fimi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.25 intracellular
-
 Cellulomonas fimi 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 130000
-
 gel filtration Cellulomonas fimi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Cellulomonas fimi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 additional information no hydrolysis of p-nitrophenyl derivatives of alpha-mannose, beta-N-acetylglucosamine, beta-glucose, beta-xylose, beta-cellobiose or beta-gentiobiose Cellulomonas fimi ?
-
 ?
3.2.1.25 p-nitrophenyl-beta-galactoside + H2O low activity Cellulomonas fimi p-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.25 p-nitrophenyl-beta-mannoside + H2O
-
 Cellulomonas fimi p-nitrophenol + beta-D-mannopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.25 beta-mannosidase 2A
-
 Cellulomonas fimi
3.2.1.25 Man2A
-
 Cellulomonas fimi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.25 55
-
-
 Cellulomonas fimi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.25 37
-
 half-life: 27 h Cellulomonas fimi
3.2.1.25 55
-
 unstable at Cellulomonas fimi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.25 additional information
-
 additional information as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the turnover number of 167 per s is only an estimate Cellulomonas fimi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 7
-
-
 Cellulomonas fimi