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Literature summary extracted from

  • Ruadze, I.D.; Zherebtsov, N.A.; Slepokurova, Y.I.; Selemenev, V.F.; Shkutina, I.V.; Stoyanova, O.F.
    Preparation and characterization of immobilized Aspergillus awamori 466 glucoamylase (2001), Appl. Biochem. Microbiol., 37, 178-183.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Aspergillus awamori
-
 beta-D-glucose + ?
-
 ?
3.2.1.3 starch + H2O Aspergillus awamori 466
-
 beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus awamori
-
-
-
3.2.1.3 Aspergillus awamori 466
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 ethanol precipitation Aspergillus awamori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 26 39 immobilized glucoamylase Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O
-
 Aspergillus awamori beta-D-glucose + ?
-
 ?
3.2.1.3 starch + H2O
-
 Aspergillus awamori 466 beta-D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 GA
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 60 70 immobilized glucoamylase Aspergillus awamori
3.2.1.3 65
-
 native glucoamylase Aspergillus awamori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 20 75 immobilized glucamylase, approx. 20% of maximal activity at 30°C, approx. 50% of maximal activity at 75°C Aspergillus awamori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4 5.5 immobilized glucoamylase Aspergillus awamori
3.2.1.3 5
-
 native glucoamylase Aspergillus awamori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 2 7 native glucoamylase, approx. 20% of maximal activity at pH 2.0 and pH 6.0, respectively, immobilized glucoamylase, approx. 30% of maximal activity at pH 2.0, approx. 40% of maximal activity at pH 6.0 Aspergillus awamori