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Literature summary extracted from

  • Serrat, M.; Bermudez, R.C.; Villa, T.G.
    Production, purification, and characterization of a polygalacturonase from a new strain of Kluyveromyces marxianus isolated from coffee wet-processing wastewater (2002), Appl. Biochem. Biotechnol., 97, 193-208.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.15 0.17
-
 polygalacturonic acid
-
 Kluyveromyces marxianus
3.2.1.15 48.8
-
 Pectin apple pectin, 75% degree of esterification Kluyveromyces marxianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 41700
-
 x * 41700, SDS-PAGE Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.15 additional information Kluyveromyces marxianus O2 and galacturonate negatively regulate enzyme synthesis, and glucose as carbon source affords better enzyme yield than lactose ?
-
 ?
3.2.1.15 additional information Kluyveromyces marxianus CCEBI 2011 O2 and galacturonate negatively regulate enzyme synthesis, and glucose as carbon source affords better enzyme yield than lactose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Kluyveromyces marxianus
-
-
-
3.2.1.15 Kluyveromyces marxianus CCEBI 2011
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15
-
 Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 219
-
-
 Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 additional information O2 and galacturonate negatively regulate enzyme synthesis, and glucose as carbon source affords better enzyme yield than lactose Kluyveromyces marxianus ?
-
 ?
3.2.1.15 additional information O2 and galacturonate negatively regulate enzyme synthesis, and glucose as carbon source affords better enzyme yield than lactose Kluyveromyces marxianus CCEBI 2011 ?
-
 ?
3.2.1.15 pectin + H2O apple pectin, 75% degree of esterification Kluyveromyces marxianus ?
-
 ?
3.2.1.15 pectin + H2O apple pectin, 75% degree of esterification Kluyveromyces marxianus CCEBI 2011 ?
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Kluyveromyces marxianus ?
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Kluyveromyces marxianus CCEBI 2011 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 41700, SDS-PAGE Kluyveromyces marxianus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 PG
-
 Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 55
-
-
 Kluyveromyces marxianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 50
-
 14 h, 50% loss of activity Kluyveromyces marxianus
3.2.1.15 55
-
 8.7 h: 50% loss of activity Kluyveromyces marxianus
3.2.1.15 60
-
 complete inactivation after 6 min Kluyveromyces marxianus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.15 1.83
-
 polygalacturonic acid
-
 Kluyveromyces marxianus
3.2.1.15 6.08
-
 Pectin apple pectin, 75% degree of esterification Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 4.5
-
-
 Kluyveromyces marxianus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 3 4.5 pH 3.0: about 50% of maximal activity, pH 4.5: optimum Kluyveromyces marxianus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 3 5 stable Kluyveromyces marxianus