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Literature summary extracted from
- A beta-glucosidase from Sclerotinia sclerotiorum: Biochemical characterization and use in oligosaccharide synthesis (2004), Appl. Biochem. Biotechnol., 112, 63-77.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Fe2+ | strong inhibition | Sclerotinia sclerotiorum |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.09 | - |
p-nitrophenyl-beta-D-glucopyranoside | - |
Sclerotinia sclerotiorum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | - |
Sclerotinia sclerotiorum | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Ca2+ | 35% activation | Sclerotinia sclerotiorum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 60000 | - |
2 * 60000, SDS-PAGE | Sclerotinia sclerotiorum |
| 3.2.1.21 | 130000 | - |
gel filtration | Sclerotinia sclerotiorum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Sclerotinia sclerotiorum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | ammonium sulfate, gel filtration, anion-exchange, gel filtration | Sclerotinia sclerotiorum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | p-nitrophenyl-beta-D-glucopyranoside + H2O | beta-Glu x also has transglucosylation activity | Sclerotinia sclerotiorum | p-nitrophenol + beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | dimer | 2 * 60000, SDS-PAGE | Sclerotinia sclerotiorum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | beta-Glu x | - |
Sclerotinia sclerotiorum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 55 | 60 | - |
Sclerotinia sclerotiorum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
- |
Sclerotinia sclerotiorum |
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Release 2023.1 (January 2023)
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