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Literature summary extracted from

  • Taylor, L.P.; Miller, K.D.
    The use of a photoactivatable kaempferol analogue to probe the role of flavonol 3-O-galactosyltransferase in pollen germination (2002), Adv. Exp. Med. Biol., 505, 41-50.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.234 expression in Escherichia coli Petunia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.234 2-(3-benzoylphenyl)-3,5,7-trihydroxychromen-4-one irreversible inhibitor, the effects of the inhibitor are mediated by UV-A light treatment, binding characteristics, kaempferol protects against inhibition Petunia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.234 0.0011
-
 kaempferol
-
 Petunia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.234 additional information localization of F3GalTase in pollen Petunia sp.
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.234 UDP-galactose + kaempferol Petunia sp. role in pollen germination, F3GalTase is a gametophyte-specific enzyme, that controls the accumulation of glycosylated flavonols in pollen UDP + kaempferol 3-O-beta-D-galactoside endogenous product in pollen, which stimulates pollen germination r
2.4.1.234 UDP-galactose + quercetin Petunia sp. role in pollen germination, F3GalTase is a gametophyte-specific enzyme, that controls the accumulation of glycosylated flavonols in pollen UDP + quercetin 3-O-beta-D-galactoside endogenous product in pollen, which stimulates pollen germination r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.234 Petunia sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.234 gametophyte gametophyte-specific enzyme Petunia sp.
-
2.4.1.234 pollen CMF Petunia sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.234 UDP-galactose + 2-(3-benzoylphenyl)-3,5,7-trihydroxychromen-4-one 20-30% of the activity with kaempferol as substrate Petunia sp. UDP + 2-(3-benzoylphenyl)-3,5,7-trihydroxychromen-4-one galactoside
-
 ?
2.4.1.234 UDP-galactose + kaempferol role in pollen germination, F3GalTase is a gametophyte-specific enzyme, that controls the accumulation of glycosylated flavonols in pollen Petunia sp. UDP + kaempferol 3-O-beta-D-galactoside endogenous product in pollen, which stimulates pollen germination r
2.4.1.234 UDP-galactose + kaempferol F3GalTase is a highly discriminating and efficient enzyme Petunia sp. UDP + kaempferol 3-O-beta-D-galactoside
-
 r
2.4.1.234 UDP-galactose + quercetin role in pollen germination, F3GalTase is a gametophyte-specific enzyme, that controls the accumulation of glycosylated flavonols in pollen Petunia sp. UDP + quercetin 3-O-beta-D-galactoside endogenous product in pollen, which stimulates pollen germination r
2.4.1.234 UDP-galactose + quercetin F3GalTase is a highly discriminating and efficient enzyme Petunia sp. UDP + quercetin 3-O-beta-D-galactoside
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.234 monomer
-
 Petunia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.234 6
-
 reverse activity Petunia sp.
2.4.1.234 8
-
 forward activity Petunia sp.